Human ALK-1/ACVRL1 ELISA Kit PicoKine®

ALK-1 ELISA kit for Human

Human ALK-1/ACVRL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACVRL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Product Info Summary

SKU: EK1518
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human ALK-1/ACVRL1 ELISA Kit PicoKine®

View all ALK-1 ELISA kits

SKU/Catalog Number

EK1518

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ALK-1/ACVRL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACVRL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ALK-1/ACVRL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1518)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D22-Q118

Sensitivity

<15 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1518 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1518 is reactive to ACVRL1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1518 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ALK-1

Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

Kit Components

Catalog Number Description Quantity
EK1518-CAP Anti-Human ACVRL1 Pre-coated 96-well strip microplate 1
EK1518-ST Human ACVRL1 Standard 2 vials, 10 ng/tube
EK1518-DA Human ACVRL1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.993.8187.537575015003000
O.D.0.1140.1520.1940.2690.4650.7851.3422.049

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ALK-1/ACVRL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11037713761113661411
Standard deviation5.8317.7171.555.8822.6973.37
CV (%)5.3%4.7%5.2%5.3%6.2%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ALK-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 111097102971015.315.2%
Sample 23773683783583708.072.1%
Sample 31376122711331161122494.027.6%
*number of samples for each test n=16.

Gene/Protein Information For ACVRL1 (Source: Uniprot.Org, NCBI)

Gene Name

ACVRL1

Full Name

Serine/threonine-protein kinase receptor R3

Weight

56.124kDa

Superfamily

protein kinase superfamily

Alternative Names

activin A receptor type II-like 1; activin A receptor, type II-like kinase 1; Activin receptor-like kinase 1; ACVRL1; ACVRLK1ORW2; ALK1; ALK-1; ALK1TGF-B superfamily receptor type I; EC 2.7.11; HHT; HHT2EC 2.7.11.30; serine/threonine-protein kinase receptor R3; SKR3; TSR-I ACVRL1 ACVRLK1, ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I activin A receptor like type 1 serine/threonine-protein kinase receptor R3|TGF-B superfamily receptor type I|activin A receptor type II-like 1|activin A receptor type IL|activin A receptor, type II-like kinase 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACVRL1, check out the ACVRL1 Infographic

ACVRL1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACVRL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Human ALK-1/ACVRL1 ELISA Kit PicoKine®

Question

Does EK1518 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Human ALK-1/ACVRL1 ELISA Kit PicoKine® (EK1518) doesn't contain native human biological materials. doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: which procedure should I follow in order to thaw whole blood sample for ALK-1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-07-26

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ALK-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-07-26

Question

Q: What is the optimal O.D. value for ALK-1 ELISA kit? I used your ALK-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain ALK-1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-01-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ALK-1 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of ALK-1 that can be declared/interpreted as positive by the above standard is 15pg/ml.

Boster Scientific Support

Answered: 2020-01-04

Question

Q: how to analyze ELISA data? I measured ALK-1 level in cell lysates.

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. King

Verified customer

Asked: 2018-12-09

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-09

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-02-23

Question

Q: if the enzyme conjugated ALK-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ALK-1 antigen?

Verified Customer

Verified customer

Asked: 2017-05-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-05-01

Question

Q: can you recommend the dilution ratio of serum samples for detection of ALK-1 in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2016-05-02

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ALK-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-05-02

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