Human Alpha 1 Antichymotrypsin OneStep ELISA Kit

Product Name

Human Alpha 1 Antichymotrypsin OneStep ELISA Kit

See all SERPINA3 products

SKU/Catalog Number

EK7018

Size

96wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Alpha 1 Antichymotrypsin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Alpha 1 Antichymotrypsin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7018)

Clonality of Antibodies

See Datasheet for details

Sensitivity

6.25 ng/ml

Assay Range

6.25-400 ng/mL

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7018 is reactive to SERPINA3 in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7018 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
Microwell plate coated with anti-ACT Polyclonal Ab	12x8x1
Alpha 1-Antichymotrypsin Standard: 8 vials (ready to use)	0.2ml
Alpha 1-Antichymotrypsin Controls : 2 vials	0.2ml
Anti-ACT Enzyme Conjugate: 1 vial (ready to use)	12ml
Incubation Buffer: 1 bottle (ready to use)	12ml
Sample Diluent: 3 bottles	3x22ml
TMB Substrate: 1 bottle (ready to use)	12ml
Stop Solution: 1 bottle (ready to use)	12ml
20X Wash concentrate: 1 bottle	25ml

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SERPINA3, ELISA Target remove.

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