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Product Info Summary
| SKU: | FEK1374 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human ANGPTL3 PicoKine® Quick ELISA Kit
See all Angiopoietin-like Protein 3/ANGPTL3 products
SKU/Catalog Number
FEK1374
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ANGPTL3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ANGPTL3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1374)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S17-E460
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1374 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1374 is reactive to ANGPTL3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1374 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-like Protein 3/ANGPTL3
ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1374-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1374-ST | Human Angiopoietin-like Protein 3/ANGPTL3 Standard | 2 | 20 ng/tube |
| FEK1374-A | Human ANGPTL3 Antibody A | 1 | 3ml |
| FEK1374-B | Human ANGPTL3 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.000 | 0.240 | 0.454 | 0.818 | 1.432 | 2.188 | 2.521 | 2.873 |
Data Example Images
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Human ANGPTL3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 712 | 1841 | 9677 | 751 | 1716 | 9563 |
| Standard deviation | 31.33 | 106.78 | 522.56 | 44.31 | 125.27 | 592.91 |
| CV (%) | 4.4% | 5.8% | 5.4% | 5.9% | 7.3% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 3/ANGPTL3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 712 | 718 | 645 | 684 | 690 | 30.35 | 4.4% |
| Sample 2 | 1841 | 2134 | 2209 | 1998 | 2046 | 130.91 | 6.4% |
| Sample 3 | 9677 | 8512 | 10354 | 8424 | 9242 | 822.52 | 8.9% |
Protein Target Info & Infographic
Gene/Protein Information For ANGPTL3 (Source: Uniprot.Org, NCBI)
Gene Name
ANGPTL3
Full Name
Angiopoietin-related protein 3
Weight
53.637kDa
Alternative Names
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2 ANGPTL3 ANG-5, ANGPT5, ANL3, FHBL2 angiopoietin like 3 angiopoietin-related protein 3|angiopoietin 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANGPTL3, check out the ANGPTL3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPTL3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ANGPTL3 PicoKine® Quick ELISA Kit (FEK1374)
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