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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0960)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L165-S406
625 pg/ml - 40,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0960 is reactive to ANGPTL4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0960 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-like Protein 4/ANGPTL4
Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.
|EK0960-CAP||Anti-Human ANGPTL4 Pre-coated 96-well strip microplate||1|
|EK0960-ST||Human ANGPTL4 Standard||2 vials, 40 ng/tube|
|EK0960-DA||Human ANGPTL4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human ANGPTL4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Angiopoietin-like Protein 4/ANGPTL4, Dilution ratio of 1:1, concentration in serum is around 1.2ng/ml.
Some articles we found to cite concentrations of Angiopoietin-like Protein 4/ANGPTL4 in samples: 24626437 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 4/ANGPTL4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For ANGPTL4 (Source: Uniprot.Org, NCBI)
Angiopoietin-related protein 4
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein ANGPTL4 ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158 angiopoietin like 4 angiopoietin-related protein 4|PPARG angiopoietin related protein|fasting-induced adipose factor|hepatic angiopoietin-related protein|hepatic fibrinogen/angiopoietin-related protein|peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ANGPTL4, check out the ANGPTL4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPTL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK0960
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