Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™

Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Product Info Summary

SKU: EK0960
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™

See all Angiopoietin-like Protein 4/ANGPTL4 products

SKU/Catalog Number

EK0960

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0960)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L165-S406

Sensitivity

<20 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0960 is reactive to ANGPTL4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0960 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0960-CAP Anti-Human ANGPTL4 Pre-coated 96-well strip microplate 1
EK0960-ST Human ANGPTL4 Standard 2 vials, 40 ng/tube
EK0960-DA Human ANGPTL4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0360.2270.4050.5560.9131.4731.6982.210

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Angiopoietin-like Protein 4/ANGPTL4, Dilution ratio of 1:1, concentration in serum is around 1.2ng/ml.

Some articles we found to cite concentrations of Angiopoietin-like Protein 4/ANGPTL4 in samples: 24626437 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11144583209201120479021489
Standard deviation73.52256.64941.479.52320.93988.49
CV (%)6.6%5.6%4.5%7.1%6.7%4.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 4/ANGPTL4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11114112212951157117272.836.2%
Sample 245834850517645404787253.925.3%
Sample 32092021440205361889520447952.154.6%
*number of samples for each test n=16.

Gene/Protein Information For ANGPTL4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BY76

Gene ID

51129

Gene Name

ANGPTL4

Full Name

Angiopoietin-related protein 4

Weight

45.214kDa

Alternative Names

Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein ANGPTL4 ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158 angiopoietin like 4 angiopoietin-related protein 4|PPARG angiopoietin related protein|fasting-induced adipose factor|hepatic angiopoietin-related protein|hepatic fibrinogen/angiopoietin-related protein|peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANGPTL4, check out the ANGPTL4 Infographic

ANGPTL4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANGPTL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine™

Question

Q: can you suggest the dilution ratio of serum samples for detection of ANGPTL4 in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

B. Kapoor

Verified customer

Asked: 2020-06-19

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ANGPTL4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-06-19

Question

Q: What is the optimal O.D. value for ANGPTL4 ELISA kit? I used your ANGPTL4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ANGPTL4 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ANGPTL4 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of ANGPTL4 that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2019-08-13

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-01

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-10-01

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis ANGPTL4 using the EK0960 Human ANGPTL4 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-07

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-04-07

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