Human ANGPTL7 ELISA Kit PicoKine™

Human ANGPTL7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANGPTL7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2035
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human ANGPTL7 ELISA Kit PicoKine™

See all Angiopoietin-like Protein 7/ANGPTL7 products

SKU/Catalog Number

EK2035

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ANGPTL7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANGPTL7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human ANGPTL7 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2035)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q27-P346

Sensitivity

<50 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2035 is reactive to ANGPTL7 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2035 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2035-CAP Anti-Human ANGPTL7 Pre-coated 96-well strip microplate 1
EK2035-ST Human ANGPTL7 Standard 2 vials, 10 ng/tube
EK2035-DA Human ANGPTL7 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0440.1090.1620.2680.4800.8281.4592.148

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Angiopoietin-like Protein 7/ANGPTL7, Dilution ratio of 1:1, concentration in serum is around 300pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ANGPTL7 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14064419071456392253
Standard deviation6.8627.6997.269.5735.15173.48
CV (%)4.9%4.3%5.1%6.6%5.5%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 7/ANGPTL7 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11401291181291297.776.0%
Sample 264466070258364742.716.6%
Sample 319072152207822152088115.205.5%
*number of samples for each test n=16.

Gene/Protein Information For ANGPTL7 (Source: Uniprot.Org, NCBI)

Uniprot ID

O43827

Gene ID

10218

Gene Name

ANGPTL7

Full Name

Angiopoietin-related protein 7

Weight

40.018kDa

Alternative Names

angiopoietin-like 7; Angiopoietin-like factor; Angiopoietin-like Protein 7; angiopoietin-related protein 7; ANGPTL7; AngX; CDT6; CDT6angiopoietin-like factor (CDT6); Cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2 ANGPTL7 AngX, CDT6, dJ647M16.1 angiopoietin like 7 angiopoietin-related protein 7|angiopoietin-like factor (CDT6)|angiopoietin-like protein 7|cornea-derived transcript 6 protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANGPTL7, check out the ANGPTL7 Infographic

ANGPTL7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANGPTL7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human ANGPTL7 ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I measured ANGPTL7 level in serum.

M. Anderson

Verified customer

Asked: 2020-09-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-09-09

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring ANGPTL7 using the EK2035 Human ANGPTL7 Picokine® ELISA Kit?

H. Jha

Verified customer

Asked: 2020-05-03

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-05-03

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-12-22

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-12-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

G. Scott

Verified customer

Asked: 2019-08-13

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-13

Question

Q: is it okay to use citrate plasma as samples in Human ANGPTL7 Picokine® ELISA Kit (Catalog # EK2035)?

D. Patel

Verified customer

Asked: 2019-01-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of ANGPTL7 causing degradation of its protein structure. ANGPTL7 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ANGPTL7 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-01-26

Question

Q: What is the optimal O.D. value for ANGPTL7 ELISA kit? I performed your ANGPTL7 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ANGPTL7 even though the O.D. values are not very high?

S. Patel

Verified customer

Asked: 2018-05-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ANGPTL7 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of ANGPTL7 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2018-05-10

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

H. Surve

Verified customer

Asked: 2018-03-02

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-02

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