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Product Info Summary
| SKU: | EK1546 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human BCAM/Cd239 ELISA Kit PicoKine®
View all BCAM/CD239 ELISA kits
SKU/Catalog Number
EK1546
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BCAM/Cd239 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BCAM/Cd239 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1546)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E32-A547
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1546 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1546 is reactive to BCAM in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1546 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BCAM/CD239
Basal cell adhesion molecule is a protein that in humans is encoded by the BCAM gene. The BCAM gene encodes a glycoprotein expressed on cell surfaces. It is a member of the immunoglobulin superfamily of receptors and cell adhesion molecules. When expressed on erythrocytes, BCAM constitutes the Lutheran (Lu) and Auberger (Au) blood group antigens. And this protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1546-CAP | Anti-Human BCAM Pre-coated 96-well strip microplate | 1 |
| EK1546-ST | Human BCAM Standard | 2 vials, 10 ng/tube |
| EK1546-DA | Human BCAM Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.067 | 0.125 | 0.175 | 0.250 | 0.450 | 0.848 | 1.489 | 2.053 |
Data Example Images
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Human BCAM PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BCAM/CD239, Dilution ratio of 1:1, concentration in serum and plasma is 2-4 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BCAM/Cd239 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 210 | 1405 | 5441 | 201 | 1535 | 5023 |
| Standard deviation | 14.07 | 74.46 | 359.1 | 15.47 | 87.49 | 356.63 |
| CV (%) | 6.7% | 5.3% | 6.6% | 7.7% | 5.7% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BCAM/CD239 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 210 | 181 | 205 | 206 | 200 | 11.41 | 5.7% |
| Sample 2 | 1405 | 1288 | 1480 | 1260 | 1358 | 88.87 | 6.5% |
| Sample 3 | 5441 | 6068 | 5199 | 5671 | 5594 | 320.17 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For BCAM (Source: Uniprot.Org, NCBI)
Gene Name
BCAM
Full Name
Basal cell adhesion molecule
Weight
67.405kDa
Alternative Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19 BCAM AU, CD239, LU, MSK19 basal cell adhesion molecule (Lutheran blood group) basal cell adhesion molecule|Auberger b antigen|B-CAM cell surface glycoprotein|B-cell adhesion molecule|F8/G253 antigen|Lutheran blood group variant LUGA|basal cell adhesion molecule (Lu and Au blood groups)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BCAM, check out the BCAM Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BCAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human BCAM/Cd239 ELISA Kit PicoKine® (EK1546)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Human BCAM/Cd239 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human CD239 Picokine® ELISA Kit (Catalog # EK1546)?
R. Yang
Verified customer
Asked: 2020-09-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD239 causing degradation of its protein structure. CD239 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD239 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-09-02
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD239 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD239 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-07-03
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD239 using the EK1546 Human CD239 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-01-28
Question
Q: if the enzyme conjugated CD239 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD239 antigen?
C. Chen
Verified customer
Asked: 2017-08-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-08-13
Question
Q: What is the optimal O.D. value for CD239 ELISA kit? I used your CD239 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD239 even though the O.D. values are not very high?
M. Gandhi
Verified customer
Asked: 2017-01-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD239 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of CD239 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2017-01-21
