Human Beta-2 Microglobulin OneStep ELISA Kit

Product Name

Human Beta-2 Microglobulin OneStep ELISA Kit

See all B2M products

SKU/Catalog Number

EK7023

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Beta-2 Microglobulin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Beta-2 Microglobulin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7023)

Clonality of Antibodies

See Datasheet for details

Sensitivity

0.625 µg/ml

Assay Range

0.625-10 µg/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7023 is reactive to B2M in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7023 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
Microwells coated Murine monoclonal anti-B2 MG antibody	12x8x1
B2MG Reference Standards: 0, 0.625, 1.25, 2.5, 5, and 10	1 ml
Sample Diluent, 100 ml.	100 ml
Enzyme Conjugate Reagent, 22 ml	22 ml
TMB Reagent (One-Step), 11 ml	11 ml
Stop Solution (1N HCl), 11 ml.	11 ml
Wash concentrate 20X: 1 bottle	25 ml

Standard Concentrations and example data

	B2MG ( g/ml)	Absorbance (450 nm)
Std 1	0	0.052
Std 2	0.625	0.377
Std 3	1.25	0.745
Std 4	2.5	1.414
Std 5	5	2.085
Std 6	10	2.942

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect B2M, ELISA Target remove.

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis