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Product Info Summary
| SKU: | EK0311 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants and serum. |
Product info
Product Name
Human BMP-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0311
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
BMP2 is a TGF-β superfamily morphogen that activates canonical BMP signaling via BMP receptors (e.g., BMPR1A) and SMAD1/5/8, driving bone/cartilage formation and broader differentiation programs (Reactome: Signaling by BMP, R-HSA-201451). Assay context: quantitative sandwich ELISA kit for Human BMP2 in bone tissue, culture supernatants, and serum with reported sensitivity 2 pg/mL (per product page); can be contextualized with other stress/repair cytokines like GDF15 (injury/inflammation response; putative combined interpretation).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BMP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0311)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q283-R396
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is monoclonal antibody from mouse.
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0311 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0311 is reactive to BMP2 in Human samples
Validated Sample Types
bone tissue, cell culture supernatants and serum.
Application Guarantee
EK0311 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP2
Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0311-CAP | Anti-Human BMP2 Pre-coated 96-well strip microplate | 1 |
| EK0311-ST | Human BMP2 Standard | 2 vials, 10 ng/tube |
| EK0311-DA | Human BMP2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.052 | 0.104 | 0.151 | 0.238 | 0.399 | 0.742 | 1.246 | 1.927 |
Data Example Images
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Human BMP-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BMP2, Dilution ratio of 1:1, concentration in serum is around 50 pg/ml..
Some articles we found to cite concentrations of BMP2 in samples: 25257151 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BMP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 55 | 250 | 1005 | 59 | 255 | 1061 |
| Standard deviation | 4.29 | 13.25 | 70.35 | 4.66 | 15.55 | 83.81 |
| CV (%) | 7.8% | 5.3% | 7% | 7.9% | 6.1% | 7.9% |
Specific Publications For Human BMP-2 ELISA Kit PicoKine® (EK0311)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Human BMP-2 ELISA Kit PicoKine®
Question
Is it possible for this kit to detect BMP-2 in human cell if the sample has BMP-2 concentration within the detection range of the kit?
Verified customer
Asked: 2022-12-12
Answer
BMP-2 in human cells are hardly to be detected according to our experience. We suggest running a pilot test.
Boster Scientific Support
Answered: 2022-12-12
Question
We are looking for the ELISA kit that can detect BMP-2 expressed in human cells or E. coli. Is it possible to use this kit for this purpose? We would like to know if this kit can use human cells and E. coli as a sample. Please advise.
Verified customer
Asked: 2022-12-09
Answer
EK0311 can not detect BMP-2 expressed in E. coli. The expression level of BMP-2 in human cells are too low to be detected.
Boster Scientific Support
Answered: 2022-12-09
Question
Is EK0311 expected to detect recombinant BMP-2 expressed in E.coli?
Verified customer
Asked: 2021-06-15
Answer
For the Human BMP-2 ELISA Kit PicoKine™ (EK0311), the expression system of standard (recombinant protein) was CHO eukaryotic expression system. Our lab hasn't detected recombinant BMP-2 expressed in E.coli and we are uncertain if the kit is able to detect it. We suggest the customer run a pilot test to see if the kit will work.
Boster Scientific Support
Answered: 2021-06-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-09-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-09-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-12
Question
Will this human BMP-2 kit cross-react significantly with the rat BMP-2? Will the rat BMP-2 kit react significantly with human BMP-2? I intend to measure rat BMP-2 and rh BMP-2 on the rat critical-size calvaria defect.
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
BMP-2 is highly conserved across species. Our BMP-2 ELISA kits for human (EK0311), mouse (EK0313) and rat (EK0312) all have very high cross reactivity with BMP-2 samples of different species.
Boster Scientific Support
Answered: 2019-11-26
Question
Q: how can I thaw whole blood sample for BMP2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-09-10
Question
Will EK0311 cross-react with Human BMP-4?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Cross-reactivity was observed to be 0.8% with 20ng/mL of recombinant human BMP-4 using EK0311.
Boster Scientific Support
Answered: 2019-06-18
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring BMP2 using the EK0311 Human BMP2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-21
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-04-21
Question
Q: if the enzyme conjugated BMP2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP2 antigen?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-27
Question
Q: What is the optimal O.D. value for BMP2 ELISA kit? I used your BMP2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BMP2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BMP2 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2018-04-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-10-25
Question
What cytokines have you tested the kit for cross-reactivity?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
We do get a lot of feedback that BMP-2 has cross-reactivity with other cytokines. According to our test, the most cross-reactive target is BMP-4 (< 1%). However, there are some detectable cross-reactivities with the following: BMP-3, -5, -6 and -7 as well as other proteins in the TGF family, such as TGF Beta 1, 2 and 3. We have tested it against Activin and a few other families but no cross-reactivity was detected.
Boster Scientific Support
Answered: 2017-09-14
Question
Is the kit responsive to: (1) properly folded active rhBMP2 homodimer, (2) improperly folded inactive rhBMP2 homodimer, (3) rhBMP2 monomer (reduced form) or (4) small aggregates of any of the preceding? Keywords: detection
Verified Customer
Verified customer
Asked: 2016-04-25
Answer
The standard in the kit is rhBMP2(CHO, Q283-R396). The kit can recognize properly folded active rhBMP2 homodimer and rhBMP2 monomer in theory, we are not clear about the other two. Our ELISA kit's coating antibody does recognize the specific sequence in the many forms of rhBMP2. However the affinity is dependent on the integrity of the tertiary structure as well as proper exposure of the sequence on the aggregate surface. If monomer and properly folded homodimer have the same local tertiary structure then our kit will be able to recognize both. However this can only be determined by a pilot experiment.
Boster Scientific Support
Answered: 2016-04-25
Question
Can EK0311 measure the recombinant BMP-2 protein expressed in E. coli?
Verified Customer
Verified customer
Asked: 2014-05-20
Answer
The expression system of the standards in EK0311 is CHO. We haven't tested the recombinant BMP-2 protein expressed in E. coli. It is recommended to run a pilot test on the recombinant BMP-2 protein expressed in E. coli.
Boster Scientific Support
Answered: 2014-05-20
