Human BMP-2 ELISA Kit PicoKine™

Human BMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BMP2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 12 publication(s).

Product Info Summary

SKU: EK0311
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: bone tissue, cell culture supernatants and serum.

Product Name

Human BMP-2 ELISA Kit PicoKine™

See all BMP2 products

SKU/Catalog Number

EK0311

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human BMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BMP2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human BMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0311)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0311 is reactive to BMP2 in Human samples

Validated Sample Types

bone tissue, cell culture supernatants and serum.

Application Guarantee

EK0311 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0311-CAP Anti-Human BMP2 Pre-coated 96-well strip microplate 1
EK0311-ST Human BMP2 Standard 2 vials, 10 ng/tube
EK0311-DA Human BMP2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0020.0260.0600.1140.3090.6081.1521.989

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect BMP2, Dilution ratio of 1:1, concentration in serum is around 50pg/ml.

Some articles we found to cite concentrations of BMP2 in samples: 25257151 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human BMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)552501005592551061
Standard deviation4.2913.2570.354.6615.5583.81
CV (%)7.8%5.3%7%7.9%6.1%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BMP2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155515851532.945.5%
Sample 225024524027525213.465.3%
Sample 3100588588595293150.365.4%
*number of samples for each test n=16.

Gene/Protein Information For BMP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P12643

Gene ID

650

Gene Name

BMP2

Full Name

Bone morphogenetic protein 2

Weight

44.702kDa

Superfamily

TGF-beta family

Alternative Names

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC BMP2 BDA2A, SSFSC, SSFSC1, BMP2 bone morphogenetic protein 2 bone morphogenetic protein 2|bone morphogenetic protein 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMP2, check out the BMP2 Infographic

BMP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0311 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect
Species: Human

Tendon-to-bone healing using an injectable calcium phosphate cement combined with bone xenograft/BMP composite
Species: Human

Effects of local delivery of BMP2, zoledronate and their combination on bone microarchitecture, biomechanics and bone turnover in osteoporotic rabbits

Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration.

Calcium Phosphate Cement with BMP-2-loaded Gelatin Microspheres Enhances Bone Healing in Osteoporosis: A Pilot Study

Wang Y, You C, Wei R, Zu J, Song C, Li J, Yan J. Biomed Res Int. 2017;2017:2971413. doi: 10.1155/2017/2971413. Epub 2017 Aug 17. Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enh...

Ma Y, Ma L, Guo Q, Zhang S. J Exp Clin Cancer Res. 2010 Jun 30;29:85. Doi: 10.1186/1756-9966-29-85. Expression Of Bone Morphogenetic Protein-2 And Its Receptors In Epithelial Ovarian Cancer And Their Influence On The Prognosis Of Ovarian Cancer Pa...

Xu Hh, Liu Sh, Guo Qf, Liu Qh, Li Xy. Braz J Med Biol Res. 2013 Sep;46(9):809-14. Doi: 10.1590/1414-431X20132929. Epub 2013 Sep 6. Osteogenesis Induced In Goat Bone Marrow Progenitor Cells By Recombinant Adenovirus Coexpressing Bone Morphogenetic ...

Qiao C, Zhang K, Jin H, Miao L, Shi C, Liu X, Yuan A, Liu J, Li D, Zheng C, Zhang G, Li X, Yang B, Sun H. Int J Nanomedicine. 2013;8:2985-95. Doi: 10.2147/Ijn.S45184. Epub 2013 Aug 13. Using Poly(Lactic-Co-Glycolic Acid) Microspheres To Encapsulat...

Tian Z, Zhu Y, Qiu J, Guan H, Li L, Zheng S, Dong X, Xiao J. J Huazhong Univ Sci Technolog Med Sci. 2012 Aug;32(4):563-70. Doi: 10.1007/S11596-012-0097-4. Epub 2012 Aug 11. Synthesis And Characterization Of Uppe-Plga-Rhbmp2 Scaffolds For Bone Rege...

Han L, Lin H, Lu X, Zhi W, Wang Kf, Meng Fz, Jiang O. Mater Sci Eng C Mater Biol Appl. 2014 Jul 1;40:1-8. Doi: 10.1016/J.Msec.2014.03.043. Epub 2014 Mar 26. Bmp2-Encapsulated Chitosan Coatings On Functionalized Ti Surfaces And Their Performance In...

Li L, Zhou G, Wang Y, Yang G, Ding S, Zhou S. Biomaterials. 2015 Jan;37:218-29. Doi: 10.1016/J.Biomaterials.2014.10.015. Epub 2014 Oct 23. Controlled Dual Delivery Of Bmp-2 And Dexamethasone By Nanoparticle-Embedded Electrospun Nanofibers For The ...

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14 Customer Q&As for Human BMP-2 ELISA Kit PicoKine™

Question

Is EK0311 expected to detect recombinant BMP-2 expressed in E.coli?

Verified customer

Asked: 2021-06-15

Answer

For the Human BMP-2 ELISA Kit PicoKine™ (EK0311), the expression system of standard (recombinant protein) was CHO eukaryotic expression system. Our lab hasn't detected recombinant BMP-2 expressed in E.coli and we are uncertain if the kit is able to detect it. We suggest the customer run a pilot test to see if the kit will work.

Boster Scientific Support

Answered: 2021-06-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-09-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-09-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-09-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-09-12

Question

Will this human BMP-2 kit cross-react significantly with the rat BMP-2? Will the rat BMP-2 kit react significantly with human BMP-2? I intend to measure rat BMP-2 and rh BMP-2 on the rat critical-size calvaria defect.

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

BMP-2 is highly conserved across species. Our BMP-2 ELISA kits for human (EK0311), mouse (EK0313) and rat (EK0312) all have very high cross reactivity with BMP-2 samples of different species.

Boster Scientific Support

Answered: 2019-11-26

Question

Q: how can I thaw whole blood sample for BMP2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-09-10

Question

Can the standard of the Human BMP-2 ELISA Kit PicoKine™ (EK0311) be purchased separately?

Verified customer

Asked: 2019-07-15

Answer

Yes, you can buy the components of our ELISA kits separately.

Boster Scientific Support

Answered: 2019-07-16

Question

Will EK0311 cross-react with Human BMP-4?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

Cross-reactivity was observed to be 0.8% with 20ng/mL of recombinant human BMP-4 using EK0311.

Boster Scientific Support

Answered: 2019-06-18

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring BMP2 using the EK0311 Human BMP2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-04-21

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-04-21

Question

Q: if the enzyme conjugated BMP2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP2 antigen?

Verified Customer

Verified customer

Asked: 2019-02-27

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-02-27

Question

Q: What is the optimal O.D. value for BMP2 ELISA kit? I used your BMP2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BMP2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BMP2 that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2018-04-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-10-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-10-25

Question

What cytokines have you tested the kit for cross-reactivity?

Verified Customer

Verified customer

Asked: 2017-09-14

Answer

We do get a lot of feedback that BMP-2 has cross-reactivity with other cytokines. According to our test, the most cross-reactive target is BMP-4 (< 1%). However, there are some detectable cross-reactivities with the following: BMP-3, -5, -6 and -7 as well as other proteins in the TGF family, such as TGF Beta 1, 2 and 3. We have tested it against Activin and a few other families but no cross-reactivity was detected.

Boster Scientific Support

Answered: 2017-09-14

Question

Is the kit responsive to: (1) properly folded active rhBMP2 homodimer, (2) improperly folded inactive rhBMP2 homodimer, (3) rhBMP2 monomer (reduced form) or (4) small aggregates of any of the preceding? Keywords: detection

Verified Customer

Verified customer

Asked: 2016-04-25

Answer

The standard in the kit is rhBMP2(CHO, Q283-R396). The kit can recognize properly folded active rhBMP2 homodimer and rhBMP2 monomer in theory, we are not clear about the other two. Our ELISA kit's coating antibody does recognize the specific sequence in the many forms of rhBMP2. However the affinity is dependent on the integrity of the tertiary structure as well as proper exposure of the sequence on the aggregate surface. If monomer and properly folded homodimer have the same local tertiary structure then our kit will be able to recognize both. However this can only be determined by a pilot experiment.

Boster Scientific Support

Answered: 2016-04-25

Question

Can EK0311 measure the recombinant BMP-2 protein expressed in E. coli?

Verified Customer

Verified customer

Asked: 2014-05-20

Answer

The expression system of the standards in EK0311 is CHO. We haven't tested the recombinant BMP-2 protein expressed in E. coli. It is recommended to run a pilot test on the recombinant BMP-2 protein expressed in E. coli.

Boster Scientific Support

Answered: 2014-05-20

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