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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk|
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Human C1S ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human C1S ELISA Kit PicoKine™ (96 Tests). Quantitate Human C1S in cell culture supernatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk. Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human C1S ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1934)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E16-D688
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1934 is reactive to C1S in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk
EK1934 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Component C1s
Complement component 1s is a protein involved in the complement system. It is mapped to 12p13.31. This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.
|EK1934-CAP||Anti-Human C1S Pre-coated 96-well strip microplate||1|
|EK1934-ST||Human C1S Standard||2 vials, 50 ng/tube|
|EK1934-DA||Human C1S Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human C1S PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Complement Component C1s, Dilution ratio of 1:5000, concentration in serum is 20-50ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human C1S ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Complement Component C1s in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For C1S (Source: Uniprot.Org, NCBI)
Complement C1s subcomponent
peptidase S1 family
basic proline-rich peptide IB-1; C1 esterase; C1S; complement C1s subcomponent; Complement component 1 subcomponent s; complement component 1, s subcomponent; Complement Component C1s; EC 3.4.21; EC 22.214.171.124; FLJ44757 C1S EDSPD2 complement C1s complement C1s subcomponent|C1 esterase|basic proline-rich peptide IB-1|complement component 1 subcomponent s|complement component 1, s subcomponent*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on C1S, check out the C1S Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for C1S: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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