Product Info Summary
SKU: | EK7030 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
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Product info
Product Name
Human CA15-3 ELISA Kit
SKU/Catalog Number
EK7030
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA15-3. 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CA15-3 ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7030)
Clonality of Antibodies
See Datasheet for details
Sensitivity
3.8U/ml
Assay Range
10-300U/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7030 is reactive to MUC1 in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7030 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Anti-Human CA15-3 Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti- CA15-3 monoclonal antibody, Polystyrene micro-well plate |
Human CA15-3 Standards (S1~S4) | 4 | 0.5ml | CA15-3 (from natural protein) (10,50,150, 300) U/ml, 0.02M PBS, 20% new-born calf serum |
HRP Conjugated anti-Human CA15-3 antibody | 1 | 11ml | HRP Conjugated anti-Human CA15-3 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody |
Sample diluent | 1 | 11ml | 0.02M PBS, 20% new-born calf serum |
Controls | 1 | 0.5ml | CA15-3 (from natural protein) U/ml, 0.02M PBS, 20% new-born calf serum |
20X Wash Buffer Concentrate | 1 | 15ml | Neutral buffer, contains 0.5% Tween-20 |
Color Developing Reagent A | 1 | 7ml | Contains 11mmol/L H2O2 |
Color Developing Reagent B | 1 | 7ml | Contains 2mmol/L TMB |
Stop Solution | 1 | 7ml | 2mol/L dilute sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Click image to see more details
Human CA15-3 ELISA Kit standard curve
Protein Target Info & Infographic
Gene/Protein Information For MUC1 (Source: Uniprot.org, NCBI)
Gene Name
MUC1
Full Name
Mucin-1
Weight
122.102kDa
Alternative Names
Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 antigen|breast carcinoma-associated antigen DF3|cancer antigen 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC1, check out the MUC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human CA15-3 ELISA Kit (EK7030)
Hello CJ!
EK7030 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Long non-coding RNA LINC01003 suppresses the development of multiple myeloma by targeting miR-33a-5p/PIM1 axis
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