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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.|
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Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CA2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0681)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: S2-K260
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0681 is reactive to CA2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.
EK0681 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carbonic Anhydrase II/CA2
CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.
|EK0681-CAP||Anti-Human CA2 Pre-coated 96-well strip microplate||1|
|EK0681-ST||Human CA2 Standard||2 vials, 50 ng/tube|
|EK0681-DA||Human CA2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CA2 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carbonic Anhydrase II/CA2, Dilution ratio of 1:10, concentration in serum is 15-50ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Carbonic Anhydrase II/CA2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CA2 (Source: Uniprot.Org, NCBI)
Carbonic anhydrase 2
alpha-carbonic anhydrase family
CA2; CAII; CA-IIEC 22.214.171.124; Car2; Carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic Anhydrase II; carbonic anhydrase IICAC; carbonic dehydratase CA2 CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282 carbonic anhydrase 2 carbonic anhydrase 2|carbonate dehydratase II|carbonic anhydrase B|carbonic anhydrase C|carbonic anhydrase II|carbonic dehydratase|epididymis luminal protein 76|epididymis secretory protein Li 282*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CA2, check out the CA2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0681 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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