Human CA3 ELISA Kit PicoKine™

Human CA3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CA3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1938
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Product Name

Human CA3 ELISA Kit PicoKine™

See all Carbonic Anhydrase III/CA3 products

SKU/Catalog Number

EK1938

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CA3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CA3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CA3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1938)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-K260

Sensitivity

<50 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1938 is reactive to CA3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK1938 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1938-CAP Anti-Human CA3 Pre-coated 96-well strip microplate 1
EK1938-ST Human CA3 Standard 2 vials, 50 ng/tube
EK1938-DA Human CA3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0450.1110.1690.2800.4710.8051.4032.142

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Carbonic Anhydrase III/CA3, Dilution ratio of 1:40, concentration in serum is around 300ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CA3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13016028245741319610824732
Standard deviation53.34373.741105.8361.99390.911335.53
CV (%)4.1%6.2%4.5%4.7%6.4%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Carbonic Anhydrase III/CA3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11301129711361156122276.836.2%
Sample 260285779655254755958394.676.6%
Sample 324574244102451721425237311332.955.6%
*number of samples for each test n=16.

Gene/Protein Information For CA3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P07451

Gene ID

761

Gene Name

CA3

Full Name

Carbonic anhydrase 3

Weight

29.557kDa

Superfamily

alpha-carbonic anhydrase family

Alternative Names

CA3; CAIII; CA-III; Car3; Carbonate dehydratase III; carbonic anhydrase 3; Carbonic Anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1 CA3 CAIII, Car3 carbonic anhydrase 3 carbonic anhydrase 3|CA-III|HEL-S-167mP|carbonate dehydratase III|carbonic anhydrase III, muscle specific|carbonic anhydrase IIII|epididymis secretory sperm binding protein Li 167mP

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CA3, check out the CA3 Infographic

CA3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CA3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Human CA3 ELISA Kit PicoKine™

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

C. Green

Verified customer

Asked: 2020-07-24

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-07-24

Question

Q: can you suggest the dilution ratio of serum samples for detection of CA3 in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-07-04

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CA3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-07-04

Question

Q: how do I analyze ELISA data? I have performed CA3 level in urine.

Verified Customer

Verified customer

Asked: 2019-11-03

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-03

Question

I want to use urine samples with EK1938, do you have suggestions on when it is the best time to collect the urine samples?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

For EK1938, Our lab technicians suggest to run a pilot test with urine samples collected in the afternoon.

Boster Scientific Support

Answered: 2019-10-10

Question

Can EK1938 be used with urine samples collected in the afternoon?

Verified customer

Asked: 2019-07-10

Answer

Our lab technicians have not tested urine samples collected in the afternoon using the Human CA3 ELISA Kit PicoKine™ (EK1938). They suggest to run a pilot test with urine samples collected in the afternoon.

Boster Scientific Support

Answered: 2019-07-11

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-04-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-04-19

Question

Q: how to thaw whole blood sample for CA3 ELISA after freezing?

B. Zhao

Verified customer

Asked: 2019-03-28

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CA3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-03-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

W. Wilson

Verified customer

Asked: 2018-12-06

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-06

Question

Q: if the enzyme conjugated CA3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CA3 antigen?

Verified Customer

Verified customer

Asked: 2014-05-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-05-29

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