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Product Info Summary
| SKU: | EK0676 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Cathepsin L ELISA Kit PicoKine®
View all Cathepsin L ELISA kits
SKU/Catalog Number
EK0676
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cathepsin L ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cathepsin L ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0676)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E113-V333
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0676 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0676 is reactive to CTSL in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0676 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cathepsin L
Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0676-CAP | Anti-Human CTSL Pre-coated 96-well strip microplate | 1 |
| EK0676-ST | Human CTSL Standard | 2 vials, 10 ng/tube |
| EK0676-DA | Human CTSL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.118 | 0.175 | 0.210 | 0.291 | 0.478 | 0.821 | 1.458 | 2.095 |
Data Example Images
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Human Cathepsin L PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cathepsin L ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 204 | 726 | 1367 | 205 | 706 | 1305 |
| Standard deviation | 10.2 | 29.76 | 66.98 | 11.07 | 36.71 | 82.21 |
| CV (%) | 5% | 4.1% | 4.9% | 5.4% | 5.2% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cathepsin L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 204 | 202 | 210 | 182 | 199 | 10.52 | 5.2% |
| Sample 2 | 726 | 748 | 779 | 754 | 751 | 18.87 | 2.5% |
| Sample 3 | 1367 | 1597 | 1607 | 1555 | 1531 | 96.95 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For CTSL (Source: Uniprot.Org, NCBI)
Gene Name
CTSL
Full Name
Cathepsin L1
Weight
37.564kDa
Superfamily
peptidase C1 family
Alternative Names
Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP CTSL CATL1, MEP, CTSL cathepsin L procathepsin L|cathepsin L1|major excreted protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CTSL, check out the CTSL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTSL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cathepsin L ELISA Kit PicoKine® (EK0676)
Hello CJ!
EK0676 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and Clinical Implications of Cysteine Cathepsins in Gallbladder Carcinoma
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human Cathepsin L ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
V. Mitchell
Verified customer
Asked: 2019-12-06
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-06
Question
Q: if the enzyme conjugated Cathepsin L antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cathepsin L antigen?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-09-04
Question
Q: how to thaw whole blood sample for Cathepsin L ELISA after freezing?
Z. Tiwari
Verified customer
Asked: 2019-03-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cathepsin L for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-30
Question
Q: how to proceed with the analysis of ELISA data? I measured Cathepsin L level in cell lysates.
B. Patel
Verified customer
Asked: 2018-07-12
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-07-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-02-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-02-25
