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Product Info Summary
| SKU: | M01589 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody
SKU/Catalog Number
M01589
BM5013 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody catalog # M01589. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01589)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AACD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cathepsin L/V/K/H
Reactive Species
M01589 is reactive to CTSL in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01589 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of Cathepsin L/V/K/H using anti-Cathepsin L/V/K/H antibody (M01589).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin L/V/K/H antigen affinity purified monoclonal antibody (Catalog # M01589) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin L/V/K/H at approximately 38 kDa. The expected band size for Cathepsin L/V/K/H is at 36,38 kDa.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Cathepsin L/V/K/H expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate;
Specific Publications For Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody (M01589)
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4 Customer Q&As for Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody
Question
Would M01589 anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
You can see on the product datasheet, M01589 anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-13
Question
We are currently using anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody M01589 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
The anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody (M01589) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-10
Question
Would anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody M01589 work for IF with prostate?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
According to the expression profile of prostate, CTSL is highly expressed in prostate. So, it is likely that anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody M01589 will work for IF with prostate.
Boster Scientific Support
Answered: 2017-11-20
Question
I see that the anti-Cathepsin L/V/K/H Rabbit Monoclonal antibody M01589 works with IF, what is the protocol used to produce the result images on the product page?
G. Brown
Verified customer
Asked: 2013-03-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-03-20
