Human CCL13/MCP4 PicoKine® Quick ELISA Kit

CCL13/MCP-4 ELISA kit, 90-minute one-step protocol

Human CCL13/MCP4 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL13/MCP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: FEK0809
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

How Quick Picokine(R) ELISA kits work: Get data in 90 minutes

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Product Name

Human CCL13/MCP4 PicoKine® Quick ELISA Kit

See all CCL13/MCP-4 products

SKU/Catalog Number

FEK0809

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CCL13/MCP4 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL13/MCP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CCL13/MCP4 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0809)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-T98

Sensitivity

<10 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0809 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0809 is reactive to CCL13 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0809 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL13/MCP-4

Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Kit Components

Catalog numberDescription QuantityVolume
FEK0809-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0809-STHuman CCL13/MCP-4 Standard 2 10 ng/tube
FEK0809-AHuman CCL13 Antibody A 1 3ml
FEK0809-BHuman CCL13 Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)07.815.631.362.5125250500
O.D.0.0540.1980.3250.5790.9921.6722.2132.565

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)19872581885271
Standard deviation1.165.3917.81.265.4415.91
CV (%)6.1%6.2%6.9%7%6.4%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL13/MCP-4 in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 119181717181.267.1%
Sample 287858882862.052.4%
Sample 325823424825124811.154.5%
*number of samples for each test n=16.

Gene/Protein Information For CCL13 (Source: Uniprot.Org, NCBI)

Gene Name

CCL13

Full Name

C-C motif chemokine 13

Weight

10.986kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13 CCL13 CKb10, MCP-4, NCC-1, NCC1, SCYA13, SCYL1 C-C motif chemokine ligand 13 C-C motif chemokine 13|CK-beta-10|chemokine (C-C motif) ligand 13|monocyte chemoattractant protein 4|monocyte chemotactic protein 4|new CC chemokine 1|small inducible cytokine subfamily A (Cys-Cys), member 13|small-inducible cytokine A13

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL13, check out the CCL13 Infographic

CCL13 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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