Human CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CCL14 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ1123
Size: For 5 plates, 96 wells each, reagents only, plates are not included
Reactive Species: Human
Application: ELISA

Product Name

Human CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all CCL14 products

SKU/Catalog Number

EZ1123

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Human CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CCL14 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1123)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T20-N93

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1123 is reactive to CCL14 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EZ1123 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ1123-CA Rabbit anti- human CCL14 polyclonal antibody (Capture Antibody) 500 µl, 4 µg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ1123-DA Biotinylated goat anti- human CCL14 polyclonal antibody (Detection Antibody) 500 µl, 7.5 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ1123-ST Lyophilized recombinant human CCL14 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001,000
O.D.0.0070.0690.1610.3590.6701.2881.9552.234

Data Example Images

Gene/Protein Information For CCL14 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16627

Gene ID

6358

Gene Name

CCL14

Full Name

C-C motif chemokine 14

Weight

10.678kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 14; CCL14; chemokine (C-C motif) ligand 14; Chemokine CC-1/CC-3; chemokine CC-3; CKB1; FLJ16015; HCC-1; HCC-1(1-74); HCC-1/HCC-3; HCC-3; hemofiltrate CC chemokine 1; MCIF; member 14; NCC2CC-1; NCC-2CKb1; new CC chemokine 2; SCYA14CC-3; Small-inducible cytokine A14 CCL14 CC-1, CC-3, CKB1, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, SCYA14, SCYL2, SY14 C-C motif chemokine ligand 14 C-C motif chemokine 14|chemokine (C-C motif) ligand 14|chemokine CC-1/CC-3|chemokine CC-3|hemofiltrate CC chemokine 1|new CC chemokine 2|small inducible cytokine subfamily A (Cys-Cys), member 14|small-inducible cytokine A14

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL14, check out the CCL14 Infographic

CCL14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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