Human CCL14/HCC-1 ELISA Kit PicoKine™

Human CCL14/HCC-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1123
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CCL14/HCC-1 ELISA Kit PicoKine™

See all CCL14 products

SKU/Catalog Number

EK1123

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CCL14/HCC-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CCL14/HCC-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1123)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T20-N93

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1123 is reactive to CCL14 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1123 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1123-CAP Anti-Human CCL14 Pre-coated 96-well strip microplate 1
EK1123-ST Human CCL14 Standard 2 vials, 10 ng/tube
EK1123-DA Human CCL14 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentratio (pg/ml)015.631.262.51252505001000
O.D.0.0070.0690.1610.3590.6701.2881.9552.234

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CCL14/HCC-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3723844540227446
Standard deviation1.9213.825.812.0815.8933.45
CV (%)5.2%5.8%5.8%5.2%7%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL14 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 137343635351.113.1%
Sample 223825025121623814.095.9%
Sample 344550152349949228.725.8%
*number of samples for each test n=16.

Gene/Protein Information For CCL14 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16627

Gene ID

6358

Gene Name

CCL14

Full Name

C-C motif chemokine 14

Weight

10.678kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 14; CCL14; chemokine (C-C motif) ligand 14; Chemokine CC-1/CC-3; chemokine CC-3; CKB1; FLJ16015; HCC-1; HCC-1(1-74); HCC-1/HCC-3; HCC-3; hemofiltrate CC chemokine 1; MCIF; member 14; NCC2CC-1; NCC-2CKb1; new CC chemokine 2; SCYA14CC-3; Small-inducible cytokine A14 CCL14 CC-1, CC-3, CKB1, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, SCYA14, SCYL2, SY14 C-C motif chemokine ligand 14 C-C motif chemokine 14|chemokine (C-C motif) ligand 14|chemokine CC-1/CC-3|chemokine CC-3|hemofiltrate CC chemokine 1|new CC chemokine 2|small inducible cytokine subfamily A (Cys-Cys), member 14|small-inducible cytokine A14

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL14, check out the CCL14 Infographic

CCL14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1123 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Guo H, Nan K, Hu T, Meng J, Hui W, Zhang X, Qin H, Sui C. Hepatol Res. 2010 Nov;40(11):1107-16. Doi: 10.1111/J.1872-034X.2010.00721.X. Epub 2010 Sep 28. Prognostic Significance Of Co-Expression Of Nm23 And P57 Protein In Hepatocellular Carcinoma.

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Have a question?

Find answers in Q&As, reviews.

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9 Customer Q&As for Human CCL14/HCC-1 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

G. Mangal

Verified customer

Asked: 2020-12-20

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-20

Question

Q: if the enzyme conjugated CCL14 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL14 antigen?

H. Babu

Verified customer

Asked: 2020-11-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-16

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-11-05

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-11-05

Question

Q: how to thaw whole blood sample for CCL14 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL14 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-03-28

Question

Can EK1123 be used in urine sample?

Verified customer

Asked: 2019-03-11

Answer

For Human CCL14/HCC-1 ELISA Kit PicoKine™ (EK1123), we have not validated this kit with urine sample. In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest customers to run pilot experiments to decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-03-11

Question

Q: how to proceed with the analysis of ELISA data? I have performed CCL14 level in plasma.

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-03-06

Question

Q: can I use citrate plasma as samples in Human CCL14 Picokine® ELISA Kit (Catalog # EK1123)?

Verified Customer

Verified customer

Asked: 2018-06-03

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CCL14 causing disruption of its protein structure. CCL14 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CCL14 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-06-03

Question

Q: What is the optimal O.D. value for CCL14 ELISA kit? I used your CCL14 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL14 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2017-04-14

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL14 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL14 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-04-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

S. Surve

Verified customer

Asked: 2015-12-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-12-25

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