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Product Info Summary
| SKU: | EK1811 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Human CD164 ELISA Kit PicoKine®
SKU/Catalog Number
EK1811
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD164 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD164 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD164 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1811)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D24-D162
Sensitivity
<50 pg/ml
Assay Range
93.8 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1811 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1811 is reactive to CD164 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1811 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD164
Sialomucin core protein 24 also known as endolyn or CD164， is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1811-CAP | Anti-Human CD164 Pre-coated 96-well strip microplate | 1 |
| EK1811-ST | Human CD164 Standard | 2 vials, 10 ng/tube |
| EK1811-DA | Human CD164 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.000 | 0.082 | 0.173 | 0.294 | 0.522 | 0.976 | 1.585 | 2.118 |
Data Example Images
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Human CD164 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD164, Dilution ratio of 1:1, concentration in serum and plasma is 1-2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD164 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 149 | 717 | 2964 | 162 | 741 | 2982 |
| Standard deviation | 8.2 | 29.4 | 168.95 | 10.04 | 48.17 | 217.7 |
| CV (%) | 5.5% | 4.1% | 5.7% | 6.2% | 6.5% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD164 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 149 | 135 | 144 | 154 | 145 | 7.01 | 4.8% |
| Sample 2 | 717 | 672 | 786 | 731 | 726 | 40.68 | 5.6% |
| Sample 3 | 2964 | 3060 | 3039 | 3034 | 3049 | 62.59 | 2.0% |
Protein Target Info & Infographic
Gene/Protein Information For CD164 (Source: Uniprot.Org, NCBI)
Gene Name
CD164
Full Name
Sialomucin core protein 24
Weight
20.917kDa
Superfamily
CD164 family
Alternative Names
CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin CD164 DFNA66, MGC-24, MGC-24v, MUC-24, endolyn CD164 molecule sialomucin core protein 24|CD164 antigen, sialomucin|multi-glycosylated core protein 24
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD164, check out the CD164 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD164: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD164 ELISA Kit PicoKine® (EK1811)
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No publications found for EK1811
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Human CD164 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CD164 ELISA kit? I performed your CD164 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD164 even though the O.D. values are not very high?
G. Wilson
Verified customer
Asked: 2019-11-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD164 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of CD164 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-11-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CD164 using the EK1811 Human CD164 Picokine® ELISA Kit?
R. Lopez
Verified customer
Asked: 2018-10-20
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-10-20
Question
Q: Can CD164 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Yang
Verified customer
Asked: 2018-08-06
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-06
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD164 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-06-23
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD164 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-06-23
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Martinez
Verified customer
Asked: 2018-03-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-08
Question
Q: how can I thaw whole blood sample for CD164 ELISA after freezing?
J. Walker
Verified customer
Asked: 2018-01-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD164 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-08
