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Product Info Summary
| SKU: | FEK0698 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human TNFRSF7/CD27 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0698
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF7/CD27 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TNFRSF7/CD27 in cell culture supernatants and serum. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human TNFRSF7/CD27 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0698)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T21-I192
Sensitivity
<1 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0698 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0698 is reactive to CD27 in Human samples
Validated Sample Types
cell culture supernatants and serum
Application Guarantee
FEK0698 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD27/TNFRSF7
CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0698-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0698-ST | Human CD27/TNFRSF7 Standard | 2 | 10 ng/tube |
| FEK0698-A | Human CD27 Antibody A | 1 | 3ml |
| FEK0698-B | Human CD27 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.064 | 0.174 | 0.272 | 0.452 | 0.782 | 1.367 | 2.134 | 2.496 |
Data Example Images
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Human CD27 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFRSF7/CD27 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 105 | 210 | 1426 | 107 | 541 | 1466 |
| Standard deviation | 6.83 | 22.95 | 51.34 | 6.21 | 30.84 | 89.43 |
| CV (%) | 6.5% | 4.5% | 3.6% | 5.8% | 5.7% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD27/TNFRSF7 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 105 | 115 | 112 | 118 | 113 | 5.51 | 4.9% |
| Sample 2 | 510 | 611 | 668 | 631 | 605 | 15.73 | 2.6% |
| Sample 3 | 1426 | 1574 | 1364 | 1462 | 1457 | 55.35 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For CD27 (Source: Uniprot.Org, NCBI)
Gene Name
CD27
Full Name
CD27 antigen
Weight
29.137kDa
Alternative Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 |CD27L receptor|T cell activation S152|T-cell activation CD27|tumor necrosis factor receptor superfamily, member 7
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD27, check out the CD27 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFRSF7/CD27 PicoKine® Quick ELISA Kit (FEK0698)
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