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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine.|
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Human CD320 / 8D6A / TCblR ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CD320 / 8D6A / TCblR ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD320 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD320 / 8D6A / TCblR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1255)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S36-Y229
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1255 is reactive to CD320 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EK1255 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1255-CAP||Anti-Human CD320 Pre-coated 96-well strip microplate||1|
|EK1255-ST||Human CD320 Standard||2 vials, 10 ng/tube|
|EK1255-DA||Human CD320 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CD320/8D6A PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD320/TCblR/8D6A, Dilution ratio of 1:1, concentration in serum is 100-360pg/ml.
Some articles we found to cite concentrations of CD320/TCblR/8D6A in samples: 22149746 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD320 / 8D6A / TCblR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD320/TCblR/8D6A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CD320 (Source: Uniprot.Org, NCBI)
8D6 antigen; 8D6A; 8D6AFDC-SM-8D6; CD320 antigenFDC-signaling molecule 8D6,8D6TCBLR; CD320 molecule; CD320; TCblR; Transcobalamin receptor CD320 8D6, 8D6A, TCBLR, TCN2R CD320 molecule CD320 antigen|8D6 antigen|FDC-SM-8D6|FDC-signaling molecule 8D6|transcobalamin 2 receptor|transcobalamin receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CD320, check out the CD320 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD320: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1255
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