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Product Info Summary
| SKU: | EK2225 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD49A ELISA Kit PicoKine®
View all Integrin alpha 1/CD49a ELISA kits
SKU/Catalog Number
EK2225
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD49A PicoKine ELISA Kit® (96 Tests). Quantitate Human CD49A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD49A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2225)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: M1-P1141
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2225 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2225 is reactive to ITGA1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2225 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Integrin alpha 1/CD49a
Integrin alpha-1 also CD49a is an integrin alpha subunit encoded in humans by the gene ITGA1. This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2225-CAP | Anti-Human ITGA1 Pre-coated 96-well strip microplate | 1 |
| EK2225-ST | Human ITGA1 Standard | 2 vials, 10 ng/tube |
| EK2225-DA | Human ITGA1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.042 | 0.087 | 0.163 | 0.395 | 0.814 | 1.241 | 1.525 | 1.842 |
Data Example Images
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Human CD49A PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD49A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 161 | 592 | 3907 | 202 | 617 | 4838 |
| Standard deviation | 9.77 | 12.31 | 100.02 | 7.84 | 35.79 | 166.91 |
| CV(%) | 6.1% | 2.1% | 2.6% | 3.9% | 5.8% | 3.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Integrin alpha 1/CD49a in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 161 | 268 | 173 | 196 | 200 | 5.83 | 2.9% |
| Sample 2 | 592 | 625 | 368 | 345 | 483 | 19.35 | 4% |
| Sample 3 | 3907 | 3743 | 3150 | 4519 | 3830 | 288.76 | 7.5% |
Protein Target Info & Infographic
Gene/Protein Information For ITGA1 (Source: Uniprot.Org, NCBI)
Gene Name
ITGA1
Full Name
Integrin alpha-1
Weight
Superfamily
integrin alpha chain family
Alternative Names
CD49 antigen-like family member A; CD49a antigen; CD49a; Integrin alpha 1; integrin, alpha 1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1integrin alpha-1 ITGA1 CD49a, VLA1 integrin subunit alpha 1 integrin alpha-1|CD49 antigen-like family member A|VLA-1|laminin and collagen receptor|very late activation protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ITGA1, check out the ITGA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ITGA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD49A ELISA Kit PicoKine® (EK2225)
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