Human CEACAM1/Cd66A ELISA Kit PicoKine®

CEACAM1 ELISA kit for Human

Human CEACAM1/Cd66A ELISA Kit PicoKine® (96 Tests). Quantitate Human CEACAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK1361
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CEACAM1/Cd66A ELISA Kit PicoKine®

View all CEACAM1 ELISA kits

SKU/Catalog Number

EK1361

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CEACAM1/Cd66A ELISA Kit PicoKine® (96 Tests). Quantitate Human CEACAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CEACAM1/Cd66A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1361)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q35-G428

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1361 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1361 is reactive to CEACAM1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1361 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CEACAM1

CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.

Kit Components

Catalog Number Description Quantity
EK1361-CAP Anti-Human CEACAM1 Pre-coated 96-well strip microplate 1
EK1361-ST Human CEACAM1 Standard 2 vials, 20 ng/tube
EK1361-DA Human CEACAM1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031262510,00000010,0000010,000010,00020,000
O.D.0.0960.2200.2860.5050.8141.2791.9702.589

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CEACAM1/Cd66A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4632835943943230109810
Standard deviation32.41150.25453.0735.42207.69529.74
CV (%)7%5.3%4.8%8.2%6.9%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CEACAM1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 146340743442543220.244.6%
Sample 228352793256426682715106.643.9%
Sample 394398061866883728635511.45.9%
*number of samples for each test n=16.

Gene/Protein Information For CEACAM1 (Source: Uniprot.Org, NCBI)

Gene Name

CEACAM1

Full Name

Carcinoembryonic antigen-related cell adhesion molecule 1

Weight

57560 MW

Superfamily

immunoglobulin superfamily

Alternative Names

Carcinoembryonic antigen-related cell adhesion molecule 1;Biliary glycoprotein 1;BGP-1;CD66a;CEACAM1;BGP, BGP1; CEACAM1 BGP, BGP1, BGPI CEA cell adhesion molecule 1 carcinoembryonic -related cell adhesion molecule 1|CD66a | CD66|carcinoembryonic related cell adhesion molecule 1|carcinoembryonic -related cell adhesion molecule 1 (biliary glycoprotein)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CEACAM1, check out the CEACAM1 Infographic

CEACAM1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CEACAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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8 Customer Q&As for Human CEACAM1/Cd66A ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-10-06

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-10-06

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-09-07

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-07

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

W. Moore

Verified customer

Asked: 2020-04-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-04-23

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Y. Moore

Verified customer

Asked: 2020-04-07

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-04-07

Question

Q: can I use citrate plasma as samples in Human CEACAM1 Picokine® ELISA Kit (Catalog # EK1361)?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CEACAM1 causing disruption of its protein structure. CEACAM1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CEACAM1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-06-18

Question

Q: how to analyze ELISA data? I have performed CEACAM1 level in serum.

Verified Customer

Verified customer

Asked: 2018-11-25

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-11-25

Question

Q: Can CEACAM1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-03-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-03-12

Question

Q: which procedure should I follow in order to thaw whole blood sample for CEACAM1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-03-29

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CEACAM1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-03-29

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