Human CES2 ELISA Kit PicoKine®

Carboxylesterase 2/CES2 ELISA kit for Human

Human CES2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CES2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1814
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Human CES2 ELISA Kit PicoKine®

View all Carboxylesterase 2/CES2 ELISA kits

SKU/Catalog Number

EK1814

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CES2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CES2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CES2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1814)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q27-L559

Sensitivity

<50 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1814 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1814 is reactive to CES2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1814 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Carboxylesterase 2/CES2

Carboxylesterase 2 is an enzyme that in humans is encoded by the CES2 gene. This gene is mapped to 16q22.1. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.

Kit Components

Catalog Number Description Quantity
EK1814-CAP Anti-Human CES2 Pre-coated 96-well strip microplate 1
EK1814-ST Human CES2 Standard 2 vials, 20 ng/tube
EK1814-DA Human CES2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0540.2480.4160.6740.9281.4381.9532.224

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Carboxylesterase 2/CES2, Dilution ratio of 1:1, concentration in serum and plasma is around 7 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CES2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4961686852153719129483
Standard deviation20.8382.6434.5730.1122.37673.3
CV (%)4.2%4.9%5.1%5.6%6.4%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Carboxylesterase 2/CES2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 149649847851249612.082.4%
Sample 216861621169219131728110.376.3%
Sample 385218662956495659078489.045.3%
*number of samples for each test n=16.

Gene/Protein Information For CES2 (Source: Uniprot.Org, NCBI)

Gene Name

CES2

Full Name

Cocaine esterase

Weight

61.807kDa

Superfamily

type-B carboxylesterase/lipase family

Alternative Names

carboxylesterase 2 (intestine, liver); Carboxylesterase 2; carboxylesterase 2EC 3.1.1.1; CE-2; CE-2PCE-2; CES2; CES2A1; EC 3.1.1; EC 3.1.1.84; hCE-2; iCE; intestinal carboxylesterase; liver carboxylesterase-2; PCE-2 CES2 CE-2A1, PCE-2, iCE, CES2 carboxylesterase 2 cocaine esterase|carboxylesterase 2 (intestine, liver)|intestinal carboxylesterase; liver carboxylesterase-2|methylumbelliferyl-acetate deacetylase 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CES2, check out the CES2 Infographic

CES2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CES2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Human CES2 ELISA Kit PicoKine®

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

K. Jha

Verified customer

Asked: 2020-06-14

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-14

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

J. Martin

Verified customer

Asked: 2019-11-19

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-19

Question

Q: Can Carboxylesterase 2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-10-05

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-05

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Carboxylesterase 2 using the EK1814 Human Carboxylesterase 2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-05-29

Question

Q: which procedure should I follow in order to thaw whole blood sample for Carboxylesterase 2 ELISA after freezing?

N. Anderson

Verified customer

Asked: 2018-10-22

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Carboxylesterase 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-10-22

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Carboxylesterase 2 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-07-22

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Carboxylesterase 2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-07-22

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