Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

Human CFD/Adipsin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFD in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1199
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

See all Complement Factor D/Adipsin products

SKU/Catalog Number

EK1199

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CFD/Adipsin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFD in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1199)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: I26-A253

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1199 is reactive to CFD in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1199 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1199-CAP Anti-Human CFD Pre-coated 96-well strip microplate 1
EK1199-ST Human CFD Standard 2 vials, 20 ng/tube
EK1199-DA Human CFD Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031262520000000020000000200000020000020000
O.D.0.0090.0930.1820.3580.7011.1561.8222.355

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)78723789080480421938576
Standard deviation44.07149.81436.5649.84144.73471.68
CV (%)6.6%%%6.6%%4.8%6.2%6.6%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Factor D/Adipsin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 178775181773277132.754.2%
Sample 22378244022612414237368.442.8%
Sample 3909510188936487399346534.025.7%
*number of samples for each test n=16.

Gene/Protein Information For CFD (Source: Uniprot.Org, NCBI)

Uniprot ID

P00746

Gene ID

1675

Gene Name

CFD

Full Name

Complement factor D

Weight

27.033kDa

Superfamily

peptidase S1 family

Alternative Names

Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN CFD ADIPSIN, ADN, DF, PFD complement factor D complement factor D|C3 convertase activator|D component of complement (adipsin)|complement factor D preproprotein|properdin factor D

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CFD, check out the CFD Infographic

CFD infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CFD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated Complement Factor D antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Complement Factor D antigen?

M. Lee

Verified customer

Asked: 2020-12-13

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-12-13

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-02-26

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

K. Scott

Verified customer

Asked: 2019-12-22

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-12-22

Question

Q: how to thaw whole blood sample for Complement Factor D ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-08-18

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Complement Factor D for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-08-18

Question

Q: how to analyze ELISA data? I have obtained Complement Factor D level in serum.

D. Zhang

Verified customer

Asked: 2018-10-07

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-10-07

Question

Q: can I use citrate plasma as samples in Human Complement Factor D Picokine® ELISA Kit (Catalog # EK1199)?

Verified Customer

Verified customer

Asked: 2018-09-16

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Complement Factor D causing disruption of its protein structure. Complement Factor D may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Complement Factor D ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-09-16

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Complement Factor D using the EK1199 Human Complement Factor D Picokine® ELISA Kit?

B. Dhawan

Verified customer

Asked: 2014-08-24

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2014-08-24

Question

Q: can you recommend the dilution ratio of serum samples for detection of Complement Factor D in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2014-04-12

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Complement Factor D ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-04-12

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