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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine.|
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Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CFD/Adipsin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFD in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1199)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: I26-A253
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1199 is reactive to CFD in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EK1199 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor D/Adipsin
Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
|EK1199-CAP||Anti-Human CFD Pre-coated 96-well strip microplate||1|
|EK1199-ST||Human CFD Standard||2 vials, 20 ng/tube|
|EK1199-DA||Human CFD Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CFD PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor D/Adipsin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CFD (Source: Uniprot.Org, NCBI)
Complement factor D
peptidase S1 family
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 18.104.22.168; PFD; Properdin factor DADIPSIN CFD ADIPSIN, ADN, DF, PFD complement factor D complement factor D|C3 convertase activator|D component of complement (adipsin)|complement factor D preproprotein|properdin factor D*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CFD, check out the CFD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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