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Product Info Summary
| SKU: | FEK0974 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit
See all Chitinase 3-like 1 products
SKU/Catalog Number
FEK0974
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human Chitinase 3-Like 1/YKL-40 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0974)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y22-T383
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0974 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0974 is reactive to CHI3L1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
FEK0974 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chitinase 3-like 1
Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0974-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0974-ST | Human Chitinase 3-like 1 Standard | 2 | 10 ng/tube |
| FEK0974-A | Human CHI3L1 Antibody A | 1 | 3ml |
| FEK0974-B | Human CHI3L1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.038 | 0.184 | 0.286 | 0.532 | 0.957 | 1.577 | 2.178 | 2.508 |
Data Example Images
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Human CHI3L1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 125 | 487 | 2196 | 117 | 486 | 2169 |
| Standard deviation | 6.75 | 24.84 | 107.6 | 7.25 | 33.05 | 127.97 |
| CV(%) | 5.4% | 5.1% | 4.9% | 6.2% | 6.8% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chitinase 3-like 1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 125 | 116 | 135 | 118 | 124 | 7.53 | 6.1% |
| Sample 2 | 487 | 475 | 488 | 471 | 480 | 25.93 | 5.4% |
| Sample 3 | 2196 | 2424 | 2016 | 1974 | 2153 | 99.02 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For CHI3L1 (Source: Uniprot.Org, NCBI)
Gene Name
CHI3L1
Full Name
Chitinase-3-like protein 1
Weight
42.625kDa
Superfamily
glycosyl hydrolase 18 family
Alternative Names
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40 CHI3L1 ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YK-40, YKL-40, YKL40, YYL-40, hCGP-39 chitinase 3 like 1 chitinase-3-like protein 1|39 kDa synovial protein|cartilage glycoprotein 39|chitinase 3-like 1 (cartilage glycoprotein-39)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CHI3L1, check out the CHI3L1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHI3L1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Chitinase 3-Like 1/YKL-40 PicoKine® Quick ELISA Kit (FEK0974)
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