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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Human CILP ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CILP ELISA Kit PicoKine™ (96 Tests). Quantitate Human CILP in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CILP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1986)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M1-R720
93.8 pg/ml - 6,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1986 is reactive to CILP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK1986 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CILP-1
Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene. It is mapped to 15q22.31. Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease.
|EK1986-CAP||Anti-Human CILP Pre-coated 96-well strip microplate||1|
|EK1986-ST||Human CILP Standard||2 vials, 10 ng/tube|
|EK1986-DA||Human CILP Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CILP PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CILP-1, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CILP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CILP-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CILP (Source: Uniprot.Org, NCBI)
Cartilage intermediate layer protein 1
cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; cartilage intermediate layer protein 1; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; Cartilage intermediate-layer protein; CILP; CILP1; CILP-1; HsT18872 CILP CILP-1, HsT18872 cartilage intermediate layer protein cartilage intermediate layer protein 1|cartilage intermediate layer protein 1 C1|cartilage intermediate layer protein 1 C2|cartilage intermediate layer protein, nucleotide pyrophosphohydrolase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CILP, check out the CILP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CILP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1986
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