Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit

CLEC3B/Tetranectin ELISA kit, 90-minute one-step protocol

Human Tetranectin/CLEC3B PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CLEC3B in cell culture supernatants and serum. Sensitivity: 10 pg/ml.

Product Info Summary

SKU: FEK1253
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and serum
Assay Time: 90 Minutes

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Product Name

Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit

See all CLEC3B/Tetranectin products

SKU/Catalog Number

FEK1253

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Tetranectin/CLEC3B PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CLEC3B in cell culture supernatants and serum. Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Tetranectin/CLEC3B PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1253)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-V202

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1253 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1253 is reactive to CLEC3B in Human samples

Validated Sample Types

cell culture supernatants and serum

Application Guarantee

FEK1253 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CLEC3B/Tetranectin

Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Kit Components

Catalog numberDescription QuantityVolume
FEK1253-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK1253-STHuman CLEC3B/Tetranectin Standard 2 20 ng/tube
FEK1253-AHuman CLEC3B Antibody A 1 3ml
FEK1253-BHuman CLEC3B Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)03126251,2502,5005,00010,00020,000
O.D.0.0020.1230.2150.3770.7031.1531.7072.189

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)4322688821341025527809
Standard deviation26.35118.27459.9231.16137.80554.43
CV(%)6.1%4.4%5.6%7.6%5.4%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CLEC3B/Tetranectin in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 143248650545146829.016.2%
Sample 226882756292930882865163.305.7%
Sample 382139092851977508393436.435.2%
*number of samples for each test n=16.

Gene/Protein Information For CLEC3B (Source: Uniprot.Org, NCBI)

Gene Name

CLEC3B

Full Name

Tetranectin

Weight

22.537kDa

Alternative Names

CLEC3B; C-type lectin domain family 3 member B; C-type lectin domain family 3, member B; DKFZp686H17246; Plasminogen kringle 4-binding protein; TETN; tetranectin (plasminogen binding protein); Tetranectin; TNA; TNAtetranectin (plasminogen-binding protein); TNtetranectin CLEC3B TN, TNA C-type lectin domain family 3 member B tetranectin|plasminogen kringle 4-binding protein|tetranectin (plasminogen-binding protein)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CLEC3B, check out the CLEC3B Infographic

CLEC3B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CLEC3B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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