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Product Info Summary
| SKU: | EK2145 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine |
Product info
Product Name
Human complement C3a ELISA Kit PicoKine®
SKU/Catalog Number
EK2145
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human C3a anaphylatoxin ELISA Kit PicoKine® (96 Tests). Quantitate Human C3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human complement C3a ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2145)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S672-R748
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<20 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2145 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2145 is reactive to C3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine
Application Guarantee
EK2145 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of C3
Complement component 3, often simply called C3, is a protein of the immune system. In humans it is encoded on chromosome 19 by a gene called C3. Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2145-CAP | Anti-Human C3 Pre-coated 96-well strip microplate | 1 |
| EK2145-ST | Human C3 Standard | 2 vials, 10 ng/tube |
| EK2145-DA | Human C3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.01 | 0.182 | 0.295 | 0.471 | 0.733 | 1.108 | 1.808 | 3.004 |
Data Example Images
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Human complement C3a PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect C3, Dilution ratio of 1:2000000, concentration in serum and plasma is 936-1300 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human complement C3a ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 63 | 213 | 1095 | 60 | 224 | 1088 |
| Standard deviation | 3.28 | 14.06 | 50.37 | 3.06 | 14.34 | 38.08 |
| CV(%) | 5.2% | 6.6% | 4.6% | 5.1% | 6.4% | 3.5% |
Specific Publications For Human complement C3a ELISA Kit PicoKine® (EK2145)
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