Human Corin PicoKine™ ELISA Kit
|Size||96wells/kit, with removable strips.|
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA).|
|Product Name||Human Corin PicoKine™ ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Size||96wells/kit, with removable strips.|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Corin. 96wells/kit, with removable strips.|
|Cite This Product||Human Corin PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1283)|
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: S67-N1042|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Antibody Clonalities||Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat
|EK1283-CAP||96-well plate precoated with anti-Human CORIN antibody||1|
|EK1283-ST||lyophilized recombinant Human CORIN standard||4ng/tube|
|EK1283-DA||biotinylated anti-Human CORIN antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human Corin PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-25min)
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Atrial natriuretic peptide-converting enzyme|
|Tissue Specificity||Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus. Detected in blood, in plasma as well as in serum (at protein level). .|
|Alternative Names||Atrial natriuretic peptide-converting enzyme;3.4.21.-;Corin;Heart-specific serine proteinase ATC2;Pro-ANP-converting enzyme;Transmembrane protease serine 10;Atrial natriuretic peptide-converting enzyme, N-terminal propeptide;Atrial natriuretic peptide-converting enzyme, activated protease fragment;Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment;Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment;Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment;CORIN;CRN, TMPRSS10;|
|Subcellular Localization||Cell membrane ; Single- pass type II membrane protein . May easily detached from the endothelial cell membrane.|
|Molecular Weight||116486 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide.|
|Research Areas||Cardiovascular, Cell Biology, Proteolysis / Ubiquitin, Proteolytic Enzymes
*You can search these to find other products in these research areas.
|Background||Atrial natriuretic peptide-converting enzyme, also called CRN or ATC2, is a protein that in humans is encoded by the CORIN gene. It is mapped to 4p12. This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. It has been found that Corin is the pro-ANP-converting enzyme and that Corin-mediated pro-ANP activation may play a role in regulating blood pressure. What’s more, Corin and ANP are essential for physiologic changes at the maternal-fetal interface. This protein may also function as a pro-brain-type natriuretic peptide convertase. Corin gene mutations which cause Corin to impair activity might be an underlying mechanism in hypertension.|
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1. Diluent the samples with the provided sample diluent buffer into 100ul.
2. Add 50ul of standard solution, when 50ul of sample will be added into a well.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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