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Product Info Summary
| SKU: | EK1983 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CRELD2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1983
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CRELD2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CRELD2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRELD2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1983)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A25-L353
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1983 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1983 is reactive to Creld2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1983 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Creld2
Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 22q13.33. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain). The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1983-CAP | Anti-Human Creld2 Pre-coated 96-well strip microplate | 1 |
| EK1983-ST | Human Creld2 Standard | 2 vials, 10 ng/tube |
| EK1983-DA | Human Creld2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 165 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.052 | 0.109 | 0.141 | 0.236 | 0.399 | 0.791 | 1.318 | 2.094 |
Data Example Images
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Human CRELD2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Creld2, Dilution ratio of 1:1, concentration in serum and plasma is around 3 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRELD2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 153 | 696 | 3699 | 209 | 874 | 4132 |
| Standard deviation | 6.43 | 42.46 | 240.44 | 11.29 | 37.58 | 231.39 |
| CV (%) | 4.2% | 6.1% | 6.5% | 5.4% | 4.3% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Creld2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 153 | 154 | 147 | 158 | 153 | 3.93 | 2.5% |
| Sample 2 | 696 | 700 | 750 | 711 | 714 | 21.35 | 2.9% |
| Sample 3 | 3699 | 3606 | 3621 | 3798 | 3681 | 76.22 | 2.0% |
Protein Target Info & Infographic
Gene/Protein Information For Creld2 (Source: Uniprot.Org, NCBI)
Gene Name
Creld2
Full Name
Protein disulfide isomerase Creld2
Weight
38.22kDa
Superfamily
CRELD family
Alternative Names
CRELD2; cysteine-rich with EGF-like domain protein 2; cysteine-rich with EGF-like domains 2; DKFZp667O055; MGC11256 Creld2|5730592L21Rik, C85758|cysteine-rich with EGF-like domains 2|protein disulfide isomerase Creld2|cysteine-rich with EGF-like domain protein 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Creld2, check out the Creld2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Creld2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CRELD2 ELISA Kit PicoKine® (EK1983)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Human CRELD2 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Creld2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Creld2 antigen?
Verified Customer
Verified customer
Asked: 2020-11-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-04
Question
Q: how can I thaw whole blood sample for Creld2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-07-22
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Creld2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-07-22
Question
Q: What is the optimal O.D. value for Creld2 ELISA kit? I used your Creld2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Creld2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-12-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Creld2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Creld2 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-12-14
Question
For the EK1983 kit, which epitope of CRELD2 the capture antibody and detection antibody on this plate is raised against?
Verified customer
Asked: 2019-11-14
Answer
For Human CRELD2 ELISA Kit PicoKine EK1983, the immunogen sequence of the capture antibody, detection antibody, and standard is A25-L353. (uniprot: Q6UXH1)
Boster Scientific Support
Answered: 2019-11-14
Question
Q: how do I analyze ELISA data? I measured Creld2 level in plasma.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Davis
Verified customer
Asked: 2019-08-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-12
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-04-14
Question
Q: can I use citrate plasma as samples in Human Creld2 Picokine® ELISA Kit (Catalog # EK1983)?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Creld2 causing degradation of its protein structure. Creld2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Creld2 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-03-26
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-19
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Creld2 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-04-26
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Creld2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-04-26
