Human CTGF ELISA Kit PicoKine™

Human CTGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTGF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2149
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human CTGF ELISA Kit PicoKine™

See all CCN2 products

SKU/Catalog Number

EK2149

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CTGF ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTGF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CTGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2149)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q27-A349

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2149 is reactive to CCN2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2149 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2149-CAP Anti-Human CCN2 Pre-coated 96-well strip microplate 1
EK2149-ST Human CCN2 Standard 2 vials, 10 ng/tube
EK2149-DA Human CCN2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.51252505001,0002,000
O.D.0.0160.0330.0650.1220.2890.5941.0521.887

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CTGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)4115369545166726
Standard deviation2.38.7234.062.759.1328.31
CV(%)5.6%5.7%4.9%6.1%5.5%3.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCN2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 141614855511.853.6%
Sample 21531671851721696.774%
Sample 369570175578673438.185.2%
*number of samples for each test n=16.

Gene/Protein Information For CCN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29279

Gene ID

1490

Gene Name

CCN2

Full Name

CCN family member 2

Weight

38.091kDa

Superfamily

CCN family

Alternative Names

CCN family member 2 CCN2 CTGF, HCS24, IGFBP8, NOV2 cellular communication network factor 2 CCN family member 2|IBP-8|IGF-binding protein 8|IGFBP-8|connective tissue growth factor|hypertrophic chondrocyte-specific protein 24|insulin-like growth factor-binding protein 8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCN2, check out the CCN2 Infographic

CCN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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