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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CTRC ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTRC in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CTRC/Chymotrypsin-C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2001)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: V30-L268
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2001 is reactive to CTRC in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK2001 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chymotrypsin C/CTRC
Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. This gene is mapped to 1p36.21. This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
|EK2001-CAP||Anti-Human CTRC Pre-coated 96-well strip microplate||1|
|EK2001-ST||Human CTRC Standard||2 vials, 10 ng/tube|
|EK2001-DA||Human CTRC Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CTRC PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Chymotrypsin C/CTRC, Dilution ratio of 1:1, concentration in serum is around 800pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CTRC/Chymotrypsin-C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Chymotrypsin C/CTRC in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CTRC (Source: Uniprot.Org, NCBI)
peptidase S1 family
Caldecrin; chymotrypsin C (caldecrin); Chymotrypsin C; chymotrypsin-C; CLCR; CLCRserum calcium decreasing factor; CTRC; EC 3.4.21; EC 22.214.171.124; ELA4; elastase 4; Elastase IV CTRC CLCR, ELA4 chymotrypsin C chymotrypsin-C|caldecrin|elastase 4|elastase IV|serum calcium decreasing factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CTRC, check out the CTRC Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTRC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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