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Product Info Summary
| SKU: | PB9127 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Annexin A1/ANXA1 Antibody Picoband®
SKU/Catalog Number
PB9127
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin A1/ANXA1 Antibody Picoband® catalog # PB9127. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin A1/ANXA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9127)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequences identity with mouse and rat Annexin A1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9127 is reactive to ANXA1 in Human, Monkey, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.7 kDa
Background of ANXA1
ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca (2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9127 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Positive Control
WB: human Hela whole cell, human K562 whole cell, monkey COS-7 whole cell, human A549 whole cell, human PC-3 whole cell, human U20S whole cell, rat spleen tissue, rat lung tissue, rat kidney tissue, rat C6 whole cell, human U-87MG whole cell
IHC: human tonsil tissue, rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (PB9127).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human U20S whole cell lysates,
Lane 7: rat spleen tissue lysates,
Lane 8: rat lung tissue lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: rat C6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (Catalog # PB9127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 39KD. The expected band size for Annexin A1/ANXA1 is at 39KD.
Click image to see more details
Western blot analysis of Annexin A1 using anti-Annexin A1 antibody (PB9127).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1 antigen affinity purified polyclonal antibody (PB9127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for Annexin A1 at approximately 35 kDa. The expected band size for Annexin A1 is at 39 kDa.
Click image to see more details
IHC analysis of Annexin A1 using anti-Annexin A1 antibody (PB9127).
Annexin A1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin A1 Antibody (PB9127) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of Annexin A1 using anti-Annexin A1 antibody (PB9127).
Annexin A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin A1 Antibody (PB9127) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Annexin A1/ANXA1 Antibody Picoband® (PB9127)
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16 Customer Q&As for Anti-Annexin A1/ANXA1 Antibody Picoband®
Question
I was wanting to use your anti-Annexin A1/ANXA1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, PB9127 anti-Annexin A1/ANXA1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-30
Question
I am interested in to test anti-Annexin A1/ANXA1 antibody PB9127 on rat liver for research purposes, then I may be interested in using anti-Annexin A1/ANXA1 antibody PB9127 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Gonzalez
Verified customer
Asked: 2020-03-27
Answer
The products we sell, including anti-Annexin A1/ANXA1 antibody PB9127, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-27
Question
Is this PB9127 anti-Annexin A1/ANXA1 antibody reactive to the isotypes of ANXA1?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The immunogen of PB9127 anti-Annexin A1/ANXA1 antibody is E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequences identity with mouse and rat Annexin A1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-24
Question
Is a blocking peptide available for product anti-Annexin A1/ANXA1 antibody (PB9127)?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We do provide the blocking peptide for product anti-Annexin A1/ANXA1 antibody (PB9127). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-04
Question
Does anti-Annexin A1/ANXA1 antibody PB9127 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of liver, ANXA1 is highly expressed in liver. So, it is likely that anti-Annexin A1/ANXA1 antibody PB9127 will work for WB with liver.
Boster Scientific Support
Answered: 2019-08-12
Question
My lab would like using your anti-Annexin A1/ANXA1 antibody for smooth muscle contraction studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your inquiry. This PB9127 anti-Annexin A1/ANXA1 antibody is tested on human tonsil tissue, hepg2 whole cell lysate, u87 whole cell lysate, panc whole cell lysate, smmc whole cell lysate, sw620 whole cell lysate, colo320 whole cell lysate, a549 whole cell lysate, jurkat whole cell lysate, rat lung tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-25
Question
We bought anti-Annexin A1/ANXA1 antibody for WB on mouth mucosa a few years ago. I am using human, and I plan to use the antibody for IHC next. I would like examining mouth mucosa as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
J. Bhatt
Verified customer
Asked: 2019-05-17
Answer
I have checked the website and datasheets of our anti-Annexin A1/ANXA1 antibody and it appears that PB9127 has been validated on human in both WB and IHC. Thus PB9127 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-17
Question
See below the WB image, lot number and protocol we used for liver using anti-Annexin A1/ANXA1 antibody PB9127. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-09
Question
Do you have a BSA free version of anti-Annexin A1/ANXA1 antibody PB9127 available?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Annexin A1/ANXA1 antibody PB9127 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Annexin A1/ANXA1 antibody PB9127. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-07
Question
I have a question about product PB9127, anti-Annexin A1/ANXA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9127 anti-Annexin A1/ANXA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-31
Question
We have observed staining in mouse liver. Are there any suggestions? Is anti-Annexin A1/ANXA1 antibody supposed to stain liver positively?
A. Edwards
Verified customer
Asked: 2018-03-27
Answer
From what I have seen in literature liver does express ANXA1. From what I have seen in Uniprot.org, ANXA1 is expressed in mouth mucosa, cervix lung, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ANXA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-03-27
Question
We were content with the WB result of your anti-Annexin A1/ANXA1 antibody. However we have observed positive staining in mouth mucosa nucleus using this antibody. Is that expected? Could you tell me where is ANXA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
From literature, mouth mucosa does express ANXA1. Generally ANXA1 expresses in nucleus. Regarding which tissues have ANXA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-01-25
Question
Does PB9127 anti-Annexin A1/ANXA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
It shows on the product datasheet, PB9127 anti-Annexin A1/ANXA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-16
Question
We are currently using anti-Annexin A1/ANXA1 antibody PB9127 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
B. Kulkarni
Verified customer
Asked: 2015-04-29
Answer
The anti-Annexin A1/ANXA1 antibody (PB9127) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-29
Question
I see that the anti-Annexin A1/ANXA1 antibody PB9127 works with WB, what is the protocol used to produce the result images on the product page?
M. Roberts
Verified customer
Asked: 2014-11-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-11-04
