Product Info Summary
SKU: | EK0739 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine®
View all CXCL13/BLC/BCA-1 ELISA kits
SKU/Catalog Number
EK0739
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL13 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0739)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V23-R94
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0739 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0739 is reactive to CXCL13 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0739 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL13/BLC/BCA-1
C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0739-CAP | Anti-Human CXCL13 Pre-coated 96-well strip microplate | 1 |
EK0739-ST | Human CXCL13 Standard | 2 vials, 10 ng/tube |
EK0739-DA | Human CXCL13 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.026 | 0.053 | 0.118 | 0.159 | 0.287 | 0.510 | 1.151 | 1.889 |
Data Example Images
Click image to see more details
Human CXCL13/BLC PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL13/BLC/BCA-1, Dilution ratio of 1:1, concentration in serum and plasma is 31-302 pg/ml..
Some articles we found to cite concentrations of CXCL13/BLC/BCA-1 in samples: 22336440 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 32 | 216 | 441 | 30 | 232 | 416 |
Standard deviation | 2.2 | 16.84 | 24.69 | 2.19 | 20.64 | 27.3%8 |
CV (%) | 6.9% | 7.8% | 5.6% | 7.3% | 8.9% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL13/BLC/BCA-1 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 32 | 31 | 36 | 31 | 32 | 2.06 | 6.4% |
Sample 2 | 216 | 244 | 229 | 223 | 228 | 10.31 | 4.5% |
Sample 3 | 441 | 424 | 416 | 416 | 424 | 10.2 | 2.4% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL13 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL13
Full Name
C-X-C motif chemokine 13
Weight
12.664kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant) CXCL13 ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13 C-X-C motif chemokine ligand 13 C-X-C motif chemokine 13|B-cell chemoattractant|B-cell-attracting chemokine 1|B-cell-homing chemokine (ligand for Burkitts lymphoma receptor-1)|B-lymphocyte chemoattractant|CXC chemokine BLC|b cell-attracting chemokine 1|b lymphocyte chemoattractant|chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)|small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)|small-inducible cytokine B13
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL13, check out the CXCL13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (EK0739)
Hello CJ!
EK0739 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Diagnostic role of CXCL13 and CSF serology in patients with neurosyphilis
Baraka, E.A., Egilla, S.A., Hamad, G.A. et al. Does serum B lymphocyte chemoattractant protein13 (CXCL13) level correlate with parameters of disease activity and severity in rheumatoid arthritis? Clinical and musculoskeletal ultrasonographic assessment. E
Species: Human
EK0739 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine®
Question
Q: can you suggest the dilution ratio of serum samples for detection of CXCL13 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
M. Lopez
Verified customer
Asked: 2020-09-17
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL13 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-09-17
Question
Q: how to analyze ELISA data? I have performed CXCL13 level in serum.
Verified Customer
Verified customer
Asked: 2020-05-02
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Krishna
Verified customer
Asked: 2019-07-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-07-22
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CXCL13 using the EK0739 Human CXCL13 Picokine® ELISA Kit?
T. Harris
Verified customer
Asked: 2018-07-30
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-07-30
Question
Q: Can CXCL13 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Mitchell
Verified customer
Asked: 2018-03-06
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-22
Question
Q: What is the optimal O.D. value for CXCL13 ELISA kit? I used your CXCL13 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CXCL13 even though the O.D. values are not very high?
M. Zhou
Verified customer
Asked: 2017-01-25
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL13 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CXCL13 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2017-01-25