Human Nectin-2/CD112 ELISA Kit PicoKine™

Human Nectin-2/CD112 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NECTIN2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1960
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human Nectin-2/CD112 ELISA Kit PicoKine™

See all Nectin-2/CD112 products

SKU/Catalog Number

EK1960

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Nectin-2/CD112 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NECTIN2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Nectin-2/CD112 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1960)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-G360

Sensitivity

<0.1 ng/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1960 is reactive to NECTIN2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1960 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1960-CAP Anti-Human NECTIN2 Pre-coated 96-well strip microplate 1
EK1960-ST Human NECTIN2 Standard 2 vials, 10 ng/tube
EK1960-DA Human NECTIN2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0430.1620.1760.2580.4490.8571.5732.181

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Nectin-2/CD112, Dilution ratio of 1:10, concentration in serum is around 4ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Nectin-2/CD112 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2111599516726417945274
Standard deviation9.9284.75289.3517.6991.49253.15
CV (%)4.7%5.3%5.6%6.7%5.1%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Nectin-2/CD112 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12111942021992016.183.0%
Sample 21599164917941735169475.384.4%
Sample 351675008507456085214234.244.4%
*number of samples for each test n=16.

Gene/Protein Information For NECTIN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q92692

Gene ID

5819

Gene Name

NECTIN2

Full Name

Nectin-2

Weight

57.742kDa

Superfamily

nectin family

Alternative Names

CD112 antigen; CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; HVEB; HVEBpoliovirus receptor-like 2; MPH; nectin 2; Nectin2; Nectin-2; poliovirus receptor-related 2 (herpesvirus entry mediator B); poliovirus receptor-related protein 2; PRR2; PRR2nectin-2; PVRL2; PVRR2poliovirus receptor related 2 NECTIN2 CD112, HVEB, PRR2, PVRL2, PVRR2 nectin cell adhesion molecule 2 nectin-2|herpesvirus entry protein B|poliovirus receptor-like 2|poliovirus receptor-related 2 (herpesvirus entry mediator B)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NECTIN2, check out the NECTIN2 Infographic

NECTIN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NECTIN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Human Nectin-2/CD112 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated CD112 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD112 antigen?

Verified Customer

Verified customer

Asked: 2020-12-07

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-12-07

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Z. Krishna

Verified customer

Asked: 2020-07-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-07-03

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-05-09

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-02-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-16

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD112 using the EK1960 Human CD112 Picokine® ELISA Kit?

D. Zhao

Verified customer

Asked: 2019-11-24

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-11-24

Question

Q: how can I thaw whole blood sample for CD112 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD112 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-06-20

Question

Q: how to proceed with the analysis of ELISA data? I have performed CD112 level in serum.

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-12-05

Question

Q: is it okay to use citrate plasma as samples in Human CD112 Picokine® ELISA Kit (Catalog # EK1960)?

C. Campbell

Verified customer

Asked: 2018-02-01

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD112 causing disruption of its protein structure. CD112 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD112 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-02-01

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