Click image to see more details
Product Info Summary
| SKU: | FEK0728 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma(heparin, EDTA, citrate). |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0728
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human CXCL5 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0728)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A37-N114
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0728 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0728 is reactive to CXCL5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma(heparin, EDTA, citrate).
Application Guarantee
FEK0728 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL5
C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0728-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0728-ST | Human CXCL5 Standard | 2 | 10 ng/tube |
| FEK0728-A | Human CXCL5 Antibody A | 1 | 3ml |
| FEK0728-B | Human CXCL5 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.022 | 0.161 | 0.306 | 0.614 | 1.143 | 1.877 | 2.081 | 2.097 |
Data Example Images
Click image to see more details
Human CXCL5/ENA-78 PicoKine Quick ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 66 | 270 | 964 | 61 | 293 | 970 |
| Standard deviation | 2.77 | 18.09 | 74.22 | 3.53 | 22.26 | 82.45 |
| CV (%) | 4.2% | 6.7% | 7.7% | 5.8% | 7.6% | 8.5% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL5 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL5
Full Name
Weight
12.0 kDa
For more info on CXCL5, check out the CXCL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
Specific Publications For Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit (FEK0728)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
0 Reviews For Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
