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Human CXCL6/GCP2 ELISA Kit PicoKine™
Human CXCL6/GCP2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. Cited in 2 publication(s).
Human CXCL6/GCP2 ELISA Kit PicoKine™ Product Summary
| SKU: | EK0359 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10 pg/ml |
| Assay Range: | 31.2 pg/ml - 2,000 pg/ml |
Overview
| Product Name | Human CXCL6/GCP2 ELISA Kit PicoKine™
See more CXCL6 products |
|---|---|
| Catalog# | EK0359 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Human CXCL6/GCP2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product | Human CXCL6/GCP2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0359) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10 pg/ml |
| Assay Range | 31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard | Expression system for standard: E.coli; Immunogen sequence: V40-N114 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For CXCL6 (Source: Uniprot.org, NCBI)
| Uniprot Id | P80162 |
|---|---|
| NCBI Gene Id | 6372 |
| Species Of This Entry | Human |
| Gene Name | CXCL6 |
| Protein Name | C-X-C motif chemokine 6 |
| Superfamily | intercrine alpha (chemokine CxC) family |
| Alternative Names | CXCL6|chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b |
| Molecular Weight | 11897 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CXCL6, check out the CXCL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CXCL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Human CXCL6/GCP2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0359-CAP | Anti-Human CXCL6 Pre-coated 96-well strip microplate | 1 |
| EK0359-ST | Human CXCL6 Standard | 2 vials, 10 ng/tube |
| EK0359-DA | Human CXCL6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0359 Human CXCL6/GCP2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 |
| O.D. | 0.011 | 0.063 | 0.120 | 0.262 | 0.569 | 1.151 | 1.683 | 2.177 |
We measured random samples of Human CXCL6/GCP2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 57 | 297 | 818 | 55 | 310 | 882 |
| Standard deviation | 2.39 | 19.3 | 39.26 | 2.53 | 20.15 | 51.15 |
| CV (%) | 4.2% | 6.5% | 4.8% | 4.6% | 6.5% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 57 | 54 | 57 | 57 | 56 | 1.29 | 2.3% |
| Sample 2 | 297 | 302 | 286 | 282 | 291 | 8.07 | 2.7% |
| Sample 3 | 818 | 857 | 830 | 896 | 850 | 29.95 | 3.5% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 2
| Li L, Man J, Zhao L. Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia. Exp Biol Med (Maywood). 2020 Nov 9:1535370220960675. doi: 10.1177/1535370220960675. Epub ahead of print. PMID: 33167688. Species: Human EK0359 usage in article: APP:ELISA, SAMPLE:MSCS,HUAECS AND THP-1 CELLS, DILUTION:NA |
PubMed ID 33167688 |
| miRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6 Qi Chen,1,2 Dan Liu,3 Zhi Hu,4 Cheng Luo,1 and Si Lin Zheng2 Onco Targets Ther. 2019; 12: 835–848. Published online 2019 Jan 25. doi: 10.2147/OTT.S184235 | PubMed ID 30774371 |
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Questions and answers from customers related to EK0359 Human CXCL6/GCP2 ELISA Kit PicoKine™
8 Related Questions
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CXCL6 in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
C. Jackson
Asked: 2020-10-30
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL6 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-10-30
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2020-03-27
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-03-27
Question
Q: can I use citrate plasma as samples in Human CXCL6 Picokine® ELISA Kit (Catalog # EK0359)?
Verified Customer
Asked: 2019-12-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CXCL6 causing disruption of its protein structure. CXCL6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL6 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2019-09-29
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-09-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2019-02-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-28
Question
Q: how to analyze ELISA data? I have performed CXCL6 level in cell lysates.
B. Dhawan
Asked: 2019-01-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-01-20
Question
Q: if the enzyme conjugated CXCL6 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL6 antigen?
C. Huang
Asked: 2018-09-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Li
Asked: 2017-06-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-06-24
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| (888) 466-3604 |
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