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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CXCL7/NAP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PPBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0729)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A59-D128
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0729 is reactive to PPBP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0729 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PPBP
Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
|EK0729-CAP||Anti-Human PPBP Pre-coated 96-well strip microplate||1|
|EK0729-ST||Human PPBP Standard||2 vials, 10 ng/tube|
|EK0729-DA||Human PPBP Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CXCL7 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PPBP, Dilution ratio of 1:400, concentration in serum is 50-150ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PPBP in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PPBP (Source: Uniprot.Org, NCBI)
Platelet basic protein
intercrine alpha (chemokine CxC) family
Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible c PPBP B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1 pro-platelet basic protein platelet basic protein|C-X-C motif chemokine 7|CXC chemokine ligand 7|beta-thromboglobulin|chemokine (C-X-C motif) ligand 7|connective tissue-activating peptide III|leukocyte-derived growth factor|low-affinity platelet factor IV|macrophage-derived growth factor|neutrophil-activating peptide 2|small inducible cytokine B7|small inducible cytokine subfamily B, member 7|thrombocidin 1|thrombocidin 2|thromboglobulin, beta-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PPBP, check out the PPBP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PPBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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