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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human Dermatopontin/DPT ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Dermatopontin/DPT ELISA Kit PicoKine™ (96 Tests). Quantitate Human DPT in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Dermatopontin/DPT ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1633)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q19-V201
93.7 pg/ml - 6,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1633 is reactive to DPT in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1633 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dermatopontin
Dermatopontin is a protein that in humans is encoded by the DPT gene. It’s an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin.
|EK1633-CAP||Anti-Human DPT Pre-coated 96-well strip microplate||1|
|EK1633-ST||Human DPT Standard||2 vials, 10 ng/tube|
|EK1633-DA||Human DPT Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Dermatopontin/DPT PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Dermatopontin, Dilution ratio of 1:10, concentration in serum is 4-10ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Dermatopontin/DPT ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Dermatopontin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For DPT (Source: Uniprot.Org, NCBI)
Dermatopontin; DPT; Eq1; TRAMP; Tyrosine-rich acidic matrix protein DPT TRAMP dermatopontin dermatopontin|tyrosine-rich acidic matrix protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DPT, check out the DPT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DPT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1633 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition
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