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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0867)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: sf21; Immunogen sequence: T32-H266
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0867 is reactive to DKK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0867 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0867-CAP||Anti-Human DKK1 Pre-coated 96-well strip microplate||1|
|EK0867-ST||Human DKK1 Standard||2 vials, 10 ng/tube|
|EK0867-DA||Human DKK1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human DKK-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Dkk-1, Dilution ratio of 1:20, concentration in serum is around 9ng/ml.
Some articles we found to cite concentrations of Dkk-1 in samples: 25941031, 25877354, 24612589 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Dkk-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For DKK1 (Source: Uniprot.Org, NCBI)
Dickkopf-related protein 1
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DKK1, check out the DKK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DKK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0867 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
El-Shayeb AF,El-Habachi NM,Mansour AR,Zaghloul MS.Serum midkine is a more sensitive predictor for hepatocellular carcinoma than Dickkopf-1 and alpha-L-fucosidase in cirrhotic HCV patients.Medicine (Baltimore).2021 Apr 30;100(17):e25112.doi:10.1097/MD.0000000000025112.PMID:33907088;PMCID:PMC8084028.
EK0867 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Qi L,Chen J,Zhou B,Xu K,Wang K,Fang Z,Shao Y,Yuan Y,Zheng S,Hu W.HomeoboxC6 promotes metastasis by orchestrating the DKK1/Wnt/β-catenin axis in right-sided colon cancer.Cell Death Dis.2021 Apr 1;12(4):337.doi:10.1038/s41419-021-03630-x.PMID:33795652.
EK0867 usage in article: APP:ELISA, SAMPLE:HCT116 CELL, DILUTIO N:NA
Huo Q,Xu C,Shao Y,Yu Q,Huang L,Liu Y,Bao H.Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.Int J Biol Sci 2021; 17(2):574-588.doi:10.7150/ijbs.52097
EK0867 usage in article: APP:ELISA, SAMPLE:OVARIAN CANCER CELL, DILUTION:NA
DKK1 in relation to HCV induced liver cirrhosis and HCV induced HCC curative resection.
Combination of serum tumor markers dickkopf-1, DCP and AFP for the diagnosis of primary hepatocellular carcinoma
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