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Human Eotaxin / CCL11 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Eotaxin. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human Eotaxin / CCL11 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0329 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, serum and plasma(citrate, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <2pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
Introduction
C1QBP having the accession number of NP_001203 binds to the globular "heads" of c1q thus inhibiting c1 activation. C1QBP interacts with a wide range of ligands and is implicated in cell signaling. C1QBP associates with C1r and C1s in order to yield the first component of the serum complement system. C1QBP protein has been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.
C1QBP is a new marker of tumor cells and tumor-associated macrophages/myeloid cells in hypoxic/metabolically deprived areas of tumors.
Mitochondrial C1QBP is a critical mediator of p14ARF-induced apoptosis.
C1QBP functions as a chemotactic factor for immature dendritic cells, and migration is mediated through ligation of both C1QBP and cC1qR/CR.
C1QBP overexpression successfully blocks mRNA accumulation from the adenovirus major late transcription unit (MLTU) and stimulates RNA polymerase II carboxy-terminal domain phosphorylation in virus-infected cells.
C1QBP binds with Hepacivirus core protein on CD8+ and CD4+ positive t-cells and inactivates lck and akt.
Overview
| Product Name | Human Eotaxin / CCL11 ELISA Kit PicoKine™
See all CCL11 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0329 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Eotaxin/CCL11 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(citrate, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Eotaxin/CCL11 tissue specificity: |
| Cite This Product | Human Eotaxin / CCL11 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0329) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma(citrate, EDTA). |
| Sensitivity | <2pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: G24-P97 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P51671 |
|---|---|
| Gene Name | CCL11 |
| Protein Name | Eotaxin |
| Subcellular Localization | Secreted. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human Eotaxin / CCL11 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0329-CAP | 96-well plate precoated with anti-Human CCL11 antibody | 1 |
| EK0329-ST | lyophilized recombinant Human CCL11 standard | 10ng/tube |
| EK0329-DA | biotinylated anti-Human CCL11 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0329 Human Eotaxin / CCL11 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.030 | 0.091 | 0.167 | 0.301 | 0.568 | 1.134 | 1.877 | 2.273 |
We measured random samples of Human Eotaxin / CCL11 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 181 | 476 | 34 | 176 | 517 |
| Standard deviation | 1.8 | 13.39 | 37.6 | 1.93 | 15.84 | 41.87 |
| CV(%) | 5% | 7.4% | 7.9% | 5.7% | 9% | 8.1% |
We measured the performance consistency of Human Eotaxin / CCL11 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 31 | 34 | 34 | 33 | 1.78 | 5.3% |
| Sample 2 | 181 | 203 | 202 | 192 | 194 | 8.9 | 4.5% |
| Sample 3 | 476 | 507 | 504 | 491 | 494 | 12.25 | 2.4% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
| Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup... | PubMed ID 23506991 |
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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