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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (citrate, EDTA).|
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Human Eotaxin / CCL11 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Eotaxin / CCL11 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL11 in cell culture supernatants, serum and plasma (citrate, EDTA). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Eotaxin / CCL11 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0329)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: G24-P97
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0329 is reactive to CCL11 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (citrate, EDTA).
EK0329 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL11/Eotaxin
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
|EK0329-CAP||Anti-Human CCL11 Pre-coated 96-well strip microplate||1|
|EK0329-ST||Human CCL11 Standard||2 vials, 10 ng/tube|
|EK0329-DA||Human CCL11 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Eotaxin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Eotaxin / CCL11 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CCL11/Eotaxin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CCL11 (Source: Uniprot.Org, NCBI)
intercrine beta (chemokine CC) family
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) CCL11 SCYA11 C-C motif chemokine ligand 11 eotaxin|chemokine (C-C motif) ligand 11|eosinophil chemotactic protein|eotaxin-1|small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CCL11, check out the CCL11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0329 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comorbidity has no impact on eosinophil inflammation in the upper airways or on severity of the sinonasal disease in patients with nasal polyps
Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome
Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
EK0329 usage in article: APP:ELISA, SAMPLE:BRONCHOALVEOLARLAVAGE FLUID, DILUTION:NA
Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup...
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