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Product Info Summary
| SKU: | EK0332 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Erythropoietin/EPO ELISA Kit PicoKine®
View all Erythropoietin/EPO ELISA kits
SKU/Catalog Number
EK0332
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Erythropoietin/EPO ELISA Kit PicoKine™ (96 Tests). Quantitate Human EPO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1mIU/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Erythropoietin/EPO ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0332)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: A28-R193
Sensitivity
<1mIU/ml
Assay Range
3.12 mIU/ml - 200 mIU/ml
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0332 is reactive to EPO in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0332 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Erythropoietin/EPO
Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0332-CAP | Anti-Human EPO Pre-coated 96-well strip microplate | 1 |
| EK0332-ST | Human EPO Standard | 2 vials, 200 mIU/tube |
| EK0332-DA | Human EPO Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (mIU/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.049 | 0.176 | 0.236 | 0.415 | 0.671 | 1.097 | 1.657 | 2.086 |
Data Example Images
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Human EPO PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Erythropoietin/EPO, For serum, dilution ratio is 1:1 and concentration is around 60 mIU/m.
For plasma, dilution ratio is 1:1 and concentration is 3 mIU/m..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Erythropoietin/EPO ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (mIU/ml) | 4 | 23 | 68 | 5 | 24 | 76 |
| Standard deviation | 0.17 | 0.99 | 2.79 | 0.33 | 1.63 | 5.47 |
| CV (%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (mIU/ml) | Lot 2 (mIU/ml) | Lot 3 (mIU/ml) | Lot 4 (mIU/ml) | Mean (mIU/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 4 | 4 | 4 | 4 | 4 | 0.00 | 0.0% |
| Sample 2 | 23 | 23 | 23 | 21 | 22 | 0.86 | 3.9% |
| Sample 3 | 68 | 73 | 76 | 65 | 70 | 4.27 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For EPO (Source: Uniprot.Org, NCBI)
Gene Name
EPO
Full Name
Erythropoietin
Weight
21.307kDa
Superfamily
EPO/TPO family
Alternative Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EPO, check out the EPO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Erythropoietin/EPO ELISA Kit PicoKine® (EK0332)
Hello CJ!
EK0332 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Toll-like receptors 2 and 4 mediate the capacity of mesenchymal stromal cells to support the proliferation and differentiation of CD34+ cells
Toll-like receptors 2 and 4 mediate the capacity of mesenchymal stromal cells to support the proliferation and differentiation of CD34⁺ cells.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Human Erythropoietin/EPO ELISA Kit PicoKine®
Question
Hello Boster: I used your product EK0332 to measures EPO levels in human serum but I have new research studies to measure rat or dog plasma. Can this kit still suitable for animal plasma?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The product EK0332 has been design to detect human EPO and been only tested and validated to human samples. We have not tested this kit for any cross-reactivity with other species.
Boster Scientific Support
Answered: 2019-12-25
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Erythropoietin using the EK0332 Human Erythropoietin Picokine® ELISA Kit?
A. Thompson
Verified customer
Asked: 2019-12-21
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-12-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
N. Edwards
Verified customer
Asked: 2019-03-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-31
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
H. Phillips
Verified customer
Asked: 2016-02-03
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-02-03
