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Product Info Summary
| SKU: | EK0337 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine®
SKU/Catalog Number
EK0337
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine® (96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0337)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: P134-L281
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0337 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0337 is reactive to FASLG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0337 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FASLG
Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0337-CAP | Anti-Human FASLG Pre-coated 96-well strip microplate | 1 |
| EK0337-ST | Human FASLG Standard | 2 vials, 10 ng/tube |
| EK0337-DA | Human FASLG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.023 | 0.084 | 0.142 | 0.267 | 0.527 | 0.912 | 1.465 | 2.273 |
Data Example Images
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Human FASL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FASLG, Dilution ratio of 1:1, concentration in serum and plasma is 40-218 pg/ml..
Some articles we found to cite concentrations of FASLG in samples: 25764498, 25541498, 20707047 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 186 | 437 | 34 | 189 | 419 |
| Standard deviation | 2.19 | 9.67 | 28.84 | 2.1 | 12.47 | 33.1 |
| CV (%) | 6.1% | 5.2% | 6.6% | 6.2% | 6.6% | 7.9% |
Specific Publications For Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine® (EK0337)
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Customer Reviews
0 Reviews For Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine®
Customer Q&As
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6 Customer Q&As for Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for Fas Ligand ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Fas Ligand for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-10-24
Question
Does EK0337 detect soluble FASL.
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
EK0337 detects the soluble FASL.
Boster Scientific Support
Answered: 2019-03-07
Question
Q: What is the optimal O.D. value for Fas Ligand ELISA kit? I used your Fas Ligand ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Fas Ligand even though the O.D. values are not very high?
D. Robinson
Verified customer
Asked: 2019-02-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Fas Ligand ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of Fas Ligand that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2019-02-18
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Fas Ligand using the EK0337 Human Fas Ligand Picokine® ELISA Kit?
M. Kapoor
Verified customer
Asked: 2018-01-29
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-01-29
Question
Hello, I wanted to know if EK0337 detects the soluble or membrane bound FASL? or both?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
EK0337 detects the soluble FASL.
Boster Scientific Support
Answered: 2018-01-09
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-04-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-04-18
