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Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 2pg/ml. Cited in 5 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Assay Range:||15.6 pg/ml - 1,000 pg/ml|
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|Product Name||Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™
See more Fas Ligand/TNFSF6 products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 2pg/ml.|
|Cite This Product||Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0337)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Assay Range||15.6 pg/ml - 1,000 pg/ml|
|Immunogen/Standard||Expression system for standard: CHO; Immunogen sequence: P134-L281|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||356|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor ligand superfamily member 6|
|Superfamily||tumor necrosis factor family|
|Alternative Names||Fas Ligand/TNFSF6|apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for FASLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0337-CAP||Anti-Human FASLG Pre-coated 96-well strip microplate||1|
|EK0337-ST||Human FASLG Standard||2 vials, 10 ng/tube|
|EK0337-DA||Human FASLG Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0337 Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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|Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases||PubMed ID 30524196|
|Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.||PubMed ID 20222173|
|Han X, Qiu L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 2009 May;234(5):595-603. Doi: 10.3181/0812-Rm-359. Epub 2009 Feb 25. Transplantation Of Sertoli-Islet Cell Aggregates Formed By Microgravi...||PubMed ID 19244542|
|Xu J, Liu H, Wu Y, Gong X, Zhou Q, Qiao F. J Obstet Gynaecol Res. 2011 Mar;37(3):187-94. Doi: 10.1111/J.1447-0756.2010.01334.X. Epub 2010 Dec 16. Proapoptotic Effect Of Metalloproteinase 9 Secreted By Trophoblasts On Endothelial Cells.||PubMed ID 21159039|
|Xiong M, Lu J, Zhao A, Xu L, Bao S, Lin Q, Yang B. Fertil Steril. 2010 May 15;93(8):2767-9. Doi: 10.1016/J.Fertnstert.2009.11.040. Epub 2010 Feb 1. Therapy With Fasl-Gene-Modified Dendritic Cells Confers A Protective Microenvironment In Murine Pre...||PubMed ID 20117771|
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