Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™

Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0337
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™

See all Fas Ligand/TNFSF6 products

SKU/Catalog Number

EK0337

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ (96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0337)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: P134-L281

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0337 is reactive to FASLG in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0337 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0337-CAP Anti-Human FASLG Pre-coated 96-well strip microplate 1
EK0337-ST Human FASLG Standard 2 vials, 10 ng/tube
EK0337-DA Human FASLG Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0150.0780.1620.2720.4940.8901.4531.940

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3618643734189419
Standard deviation2.199.6728.842.112.4733.1
CV (%)6.1%5.2%6.6%6.2%6.6%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Fas Ligand/TNFSF6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136414134383.088.1%
Sample 21861971921981934.762.4%
Sample 343749544650747130.216.4%
*number of samples for each test n=16.

Gene/Protein Information For FASLG (Source: Uniprot.Org, NCBI)

Uniprot ID

P48023

Gene ID

356

Gene Name

FASLG

Full Name

Tumor necrosis factor ligand superfamily member 6

Weight

31.485kDa

Superfamily

tumor necrosis factor family

Alternative Names

apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6 FASLG ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A Fas ligand tumor necrosis factor ligand superfamily member 6|CD95 ligand|Fas ligand (TNF superfamily, member 6)|apoptosis (APO-1) antigen ligand 1|apoptosis antigen ligand|fas antigen ligand|mutant tumor necrosis factor family member 6|tumor necrosis factor ligand 1A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FASLG, check out the FASLG Infographic

FASLG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FASLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0337 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases

Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.

Han X, Qiu L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 2009 May;234(5):595-603. Doi: 10.3181/0812-Rm-359. Epub 2009 Feb 25. Transplantation Of Sertoli-Islet Cell Aggregates Formed By Microgravi...

Xu J, Liu H, Wu Y, Gong X, Zhou Q, Qiao F. J Obstet Gynaecol Res. 2011 Mar;37(3):187-94. Doi: 10.1111/J.1447-0756.2010.01334.X. Epub 2010 Dec 16. Proapoptotic Effect Of Metalloproteinase 9 Secreted By Trophoblasts On Endothelial Cells.

Xiong M, Lu J, Zhao A, Xu L, Bao S, Lin Q, Yang B. Fertil Steril. 2010 May 15;93(8):2767-9. Doi: 10.1016/J.Fertnstert.2009.11.040. Epub 2010 Feb 1. Therapy With Fasl-Gene-Modified Dendritic Cells Confers A Protective Microenvironment In Murine Pre...

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6 Customer Q&As for Human FASL / CD95LG / CD95 Ligand ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for Fas Ligand ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Fas Ligand for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-10-24

Question

Does EK0337 detect soluble FASL.

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

EK0337 detects the soluble FASL.

Boster Scientific Support

Answered: 2019-03-07

Question

Q: What is the optimal O.D. value for Fas Ligand ELISA kit? I used your Fas Ligand ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Fas Ligand even though the O.D. values are not very high?

D. Robinson

Verified customer

Asked: 2019-02-18

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Fas Ligand ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of Fas Ligand that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2019-02-18

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Fas Ligand using the EK0337 Human Fas Ligand Picokine® ELISA Kit?

M. Kapoor

Verified customer

Asked: 2018-01-29

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-01-29

Question

Hello, I wanted to know if EK0337 detects the soluble or membrane bound FASL? or both?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

EK0337 detects the soluble FASL.

Boster Scientific Support

Answered: 2018-01-09

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-04-18

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-04-18

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