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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk.|
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Human FLRG/FSTL3 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FLRG/FSTL3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FSTL3 in cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FLRG/FSTL3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0869)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M27-V263
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0869 is reactive to FSTL3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk.
EK0869 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Follistatin-related Gene Protein/FLRG/Fstl3
FSTL3 (Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.
|EK0869-CAP||Anti-Human FSTL3 Pre-coated 96-well strip microplate||1|
|EK0869-ST||Human FSTL3 Standard||2 vials, 10 ng/tube|
|EK0869-DA||Human FSTL3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FLRG/FSTL3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FLRG/FSTL3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Follistatin-related Gene Protein/FLRG/Fstl3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FSTL3 (Source: Uniprot.Org, NCBI)
Follistatin-related protein 3
FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3 FSTL3 FLRG, FSRP follistatin like 3 follistatin-related protein 3|follistatin-like 3 (secreted glycoprotein)|follistatin-like protein 3|follistatin-related gene protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FSTL3, check out the FSTL3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FSTL3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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