Human FLRG/FSTL3 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FLRG/FSTL3. 96wells/kit, with removable strips.
Human FLRG/FSTL3 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA), urine and human milk.|
|Sample Volume:||100μl per well|
|Product Name||Human FLRG/FSTL3 ELISA Kit PicoKine™
See all Follistatin-related Gene Protein/FLRG/Fstl3 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Follistatin-Related Gene Protein/FSTL3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA), urine and human milk. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Follistatin-Related Gene Protein/FSTL3 tissue specificity: Expressed in a wide range of tissues. .|
|Cite This Product||Human FLRG/FSTL3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0869)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA), urine and human milk.|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: M27-V263|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For FSTL3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10272|
|Species Of This Entry||Human|
|Protein Name||Follistatin-related protein 3|
|Alternative Names||Follistatin-related Gene Protein/FLRG/Fstl3|FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3|
|Post Tranlational Modifications||Phosphorylation; Glycosylation|
|Subcellular Localization||[Isoform 1]: Secreted.; [Isoform 2]: Nucleus. Although alternative initiation has been demonstrated and resulted in different localization, the major source of nuclear FSTL3 appears not to depend on translation initiation at Met-27 according to.|
|Gene Location||19p13.3, on Chromasome 19, gene sequence: NC_000019.10 (676392..683385)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FSTL3 (Source: Uniprot.org, NCBI)
|Protein Function||Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.|
|Tissue Specificity||Expressed in a wide range of tissues.|
|Related Diseases||Infertility; Fibrosis; Heart Failure; Diabetes Mellitus; Hypertrophy; Inflammation; Gestational Diabetes; Obesity; Hyperplasia; Pregnancy Complications; Hypoxia; Pituitary Diseases; Impaired Glucose Tolerance; Endometrial Polyp; Pre-eclampsia; Insulin Resistance; Myocardial Infarction; Learning Disorders; Malnutrition; Atresia|
|Related Pathways||Pathogenesis; Cell Proliferation; Glucose Homeostasis; Secretion; Transport; Spermatogenesis; Decidualization; Cell Adhesion; Intracellular Transport; Insulin Secretion; Mitosis; Fertilization; Aging; Localization; Regeneration; Flight; Translation; Tissue Remodeling; Fibroblast Activation; Wound Healing|
|Background||FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.|
|Scientific References||PMID: 9671416 by Hayette S., et al. FLRG (follistatin-related gene), a new target of chromosomal rearrangement in malignant blood disorders.|
PMID: 11459787 by Tortoriello D.V., et al. Human follistatin-related protein: a structural homologue of follistatin with nuclear localization.
Kit Components And Assay QC Data Details Of Human FLRG/FSTL3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0869-CAP||96-well plate precoated with anti-Human Follistatin-related Gene Protein/FLRG/Fstl3 antibody||1|
|EK0869-ST||lyophilized recombinant Human Follistatin-related Gene Protein/FLRG/Fstl3 standard||10ng/tube|
|EK0869-DA||biotinylated anti-Human Follistatin-related Gene Protein/FLRG/Fstl3 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0869 Human FLRG/FSTL3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human FLRG/FSTL3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human FLRG/FSTL3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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