Product Info Summary
SKU: | EK7079 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum and Plasma |
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Product info
Product Name
Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit
See all Kallikrein 3/PSA products
SKU/Catalog Number
EK7079
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles.
Cite This Product
Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7079)
Clonality of Antibodies
See Datasheet for details
Sensitivity
1 ng/ml
Assay Range
1-25 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7079 is reactive to KLK3 in Human samples
Validated Sample Types
Serum and Plasma
Application Guarantee
EK7079 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity |
1. Microwells coated with Streptavidin | 12x8x1 |
2. f-PSA Standard: 6 vials (ready to use) | 0.5 ml |
3. f-PSA Conjugate Reagent: 1 bottle (ready to use) | 12 ml |
4. TMB Substrate: 1 bottle (ready to use) | 12 ml |
5. Stop Solution: 1 bottle (ready to use) | 12 ml |
6. 20X Wash concentrate: 1 bottle | 25 ml |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
f-PSA (ng/ml) | 0 | 0.5 | 1.0 | 2.5 | 5.0 | 10.0 |
Absorbance (450 nm) | 0.01 | 0.19 | 0.39 | 0.75 | 1.55 | 2.3297 |
Data Example Images
Click image to see more details
Boster Kit Box
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Kallikrein 3/PSA, ELISA Target remove.
Protein Target Info & Infographic
Gene/Protein Information For KLK3 (Source: Uniprot.org, NCBI)
Gene Name
KLK3
Full Name
Prostate-specific antigen
Weight
28.741kDa
Superfamily
peptidase S1 family
Alternative Names
APS seminin; EC 3.4.21; EC 3.4.21.77; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin KLK3 APS, KLK2A1, PSA, hK3 kallikrein related peptidase 3 prostate-specific antigen|P-30 antigen|gamma-seminoprotein|kallikrein-3|semenogelase|seminin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK3, check out the KLK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit (EK7079)
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