Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit

Kallikrein 3/PSA ELISA kit

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Product Info Summary

SKU: EK7079
Size: 96 wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum and Plasma

Product Name

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit

See all Kallikrein 3/PSA products

SKU/Catalog Number

EK7079

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles.

Cite This Product

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7079)

Clonality of Antibodies

See Datasheet for details

Sensitivity

1 ng/ml

Assay Range

1-25 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7079 is reactive to KLK3 in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7079 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantity
1. Microwells coated with Streptavidin12x8x1
2. f-PSA Standard: 6 vials (ready to use)0.5 ml
3. f-PSA Conjugate Reagent: 1 bottle (ready to use)12 ml
4. TMB Substrate: 1 bottle (ready to use)12 ml
5. Stop Solution: 1 bottle (ready to use)12 ml
6. 20X Wash concentrate: 1 bottle25 ml

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

f-PSA (ng/ml) 00.51.02.55.010.0
Absorbance (450 nm)0.010.190.390.751.552.3297

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Kallikrein 3/PSA, ELISA Target remove.

Gene/Protein Information For KLK3 (Source: Uniprot.org, NCBI)

Gene Name

KLK3

Full Name

Prostate-specific antigen

Weight

28.741kDa

Superfamily

peptidase S1 family

Alternative Names

APS seminin; EC 3.4.21; EC 3.4.21.77; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin KLK3 APS, KLK2A1, PSA, hK3 kallikrein related peptidase 3 prostate-specific antigen|P-30 antigen|gamma-seminoprotein|kallikrein-3|semenogelase|seminin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KLK3, check out the KLK3 Infographic

KLK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KLK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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