Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit

Product Name

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit

See all KLK3 products

SKU/Catalog Number

EK7079

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles.

Cite This Product

Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7079)

Clonality of Antibodies

See Datasheet for details

Sensitivity

1 ng/ml

Assay Range

1-25 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7079 is reactive to KLK3 in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7079 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
1. Microwells coated with Streptavidin	12x8x1
2. f-PSA Standard: 6 vials (ready to use)	0.5 ml
3. f-PSA Conjugate Reagent: 1 bottle (ready to use)	12 ml
4. TMB Substrate: 1 bottle (ready to use)	12 ml
5. Stop Solution: 1 bottle (ready to use)	12 ml
6. 20X Wash concentrate: 1 bottle	25 ml

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

f-PSA (ng/ml)	0	0.5	1.0	2.5	5.0	10.0
Absorbance (450 nm)	0.01	0.19	0.39	0.75	1.55	2.3297

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect KLK3, ELISA Target remove.

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis