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Product Info Summary
| SKU: | EK0916 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and cell lysates. |
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Product info
Product Name
Human Furin ELISA Kit PicoKine®
SKU/Catalog Number
EK0916
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Furin ELISA Kit PicoKine™ (96 Tests). Quantitate Human FURIN in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Furin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0916)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D108-E715
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0916 is reactive to FURIN in Human samples
Validated Sample Types
cell culture supernatants and cell lysates.
Application Guarantee
EK0916 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FURIN
Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0916-CAP | Anti-Human FURIN Pre-coated 96-well strip microplate | 1 |
| EK0916-ST | Human FURIN Standard | 2 vials, 20 ng/tube |
| EK0916-DA | Human FURIN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.015 | 0.055 | 0.096 | 0.177 | 0.346 | 0.704 | 1.343 | 1.882 |
Data Example Images
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Human Furin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FURIN, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.
Some articles we found to cite concentrations of FURIN in samples: 22186789 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Furin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 417 | 2751 | 82701 | 457 | 2701 | 7870 |
| Standard deviation | 21.68 | 143.05 | 464.4 | 28.79 | 148.55 | 448.59 |
| CV (%) | 5.2% | 5.2% | 5.6% | 5.5%% | 5.5% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FURIN in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 417 | 420 | 397 | 384 | 404 | 14.77 | 3.6% |
| Sample 2 | 2751 | 2551 | 2738 | 2985 | 2756 | 153.96 | 5.5% |
| Sample 3 | 8293 | 8993 | 7686 | 9246 | 8554 | 610.96 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For FURIN (Source: Uniprot.Org, NCBI)
Uniprot ID
P09958
Gene ID
5045
Gene Name
FURIN
Full Name
Furin
Weight
86.678kDa
Superfamily
peptidase S8 family
Alternative Names
Dibasic-processing enzyme; EC 3.4.21; EC 3.4.21.75; FUR; FURdibasic processing enzyme; furin (paired basic amino acid cleaving enzyme); Furin; furin, membrane associated receptor protein; PACE; PACEFES upstream region; paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein); Paired basic amino acid residue-cleaving enzyme; PC; PCSK3; PCSK3furin; proprotein convertase subtilisin/kexin type 3; SPC1 FURIN FUR, PACE, PCSK3, SPC1 furin, paired basic amino acid cleaving enzyme furin|FES upstream region|dibasic processing enzyme|furin, membrane associated receptor protein|paired basic amino acid residue-cleaving enzyme|proprotein convertase subtilisin/kexin 3|proprotein convertase subtilisin/kexin type 3|subtilisin-like proprotein convertase 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FURIN, check out the FURIN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FURIN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human Furin ELISA Kit PicoKine® (EK0916)
No publications found for EK0916
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human Furin ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-06-19
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-19
Question
Q: What is the optimal O.D. value for FURIN ELISA kit? I performed your FURIN ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain FURIN even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-05-16
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FURIN ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of FURIN that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-05-16
Question
Q: if the enzyme conjugated FURIN antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FURIN antigen?
D. Li
Verified customer
Asked: 2020-04-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-11
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-06-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-06-23
Question
Q: can you recommend the dilution ratio of serum samples for detection of FURIN in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FURIN ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-18
Question
Q: which procedure should I follow in order to thaw whole blood sample for FURIN ELISA after freezing?
R. Garcia
Verified customer
Asked: 2018-02-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FURIN for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-02-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Roberts
Verified customer
Asked: 2017-11-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-11-19
