Introduction
EED is a part of the Polycomb-group family whose members form multimeric protein complexe that are involved in preserving the transcriptional repressive state of genes over successive cell generations. EED mediates repression of gene activity through histone deacetylation, and acts as a specific regulator of integrin function. EED protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin ?7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins.
Overview
| Product Name |
Human Furin ELISA Kit PicoKine™
See all FURIN primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0916 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human FURIN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and cell lysates. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. FURIN tissue specificity: Seems to be expressed ubiquitously. |
| Cite This Product |
Human Furin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0916)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates and cell lysates. |
| Sensitivity |
<10pg/ml |
| Assay Range |
312pg/ml-20,000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: D108-E715 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For FURIN (Source: Uniprot.org, NCBI)
| Uniprot Id | P09958 |
|---|
| NCBI Gene Id | 5045 |
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| Species Of This Entry | Human |
|---|
| Gene Name | FURIN |
|---|
| Protein Name | Furin |
|---|
| Superfamily | peptidase S8 family |
|---|
| Alternative Names | FURIN|Dibasic-processing enzyme; EC 3.4.21; EC 3.4.21.75; FUR; FURdibasic processing enzyme; furin (paired basic amino acid cleaving enzyme); Furin; furin, membrane associated receptor protein; PACE; PACEFES upstream region; paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein); Paired basic amino acid residue-cleaving enzyme; PC; PCSK3; PCSK3furin; proprotein convertase subtilisin/kexin type 3; SPC1 |
|---|
| Molecular Weight | 86678 |
|---|
| Post Tranlational Modifications | Cleavage; Glycosylation; Phosphorylation; Amidation; Ribosylation; Biotinylation; Reduction; Deglycosylation; Dephosphorylation; Acylation; Deamidation; Gamma-carboxylation; Oxidation; Sulphation; Ubiquitination; Iodination; Acetylation; Demethylation; Hydroxylation; Gamma-cleavage |
|---|
| Subcellular Localization | Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Secreted. Endosome membrane; Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface (PubMed:9412467, PubMed:11799113). Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin (PubMed:11799113). |
|---|
| Gene Location | 15q26.1, on Chromasome 15, gene sequence: NC_000015.10 (90868588..90883458) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FURIN (Source: Uniprot.org, NCBI)
| Protein Function | Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. |
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| Tissue Specificity | Seems to be expressed ubiquitously. |
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| Interacting Proteins | MMP14; MMP28; MSTN |
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| Research Areas | Golgi Apparatus Markers; Stem Cell Signaling Pathway |
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| Related Diseases | Neoplasms; Malignant Neoplasms; Anthrax Disease; Carcinoma; Vaccinia; Tissue Adhesions; Hiv Infections; Influenza; Nervousness; Cell Invasion; Immunologic Deficiency Syndromes; Diabetes Mellitus; Pituitary Diseases; Alzheimer's Disease; Von Willebrand Disease; Inflammation; Influenza In Birds; Neoplasm Metastasis; Infective Disorder; Virus Diseases |
|---|
| Related Pathways | Secretion; Secretory Pathway; Localization; Proteolysis; Transport; Glycosylation; Endocytosis; Pathogenesis; Constitutive Secretory Pathway; Membrane Fusion; Translation; Cell Growth; Virulence; Angiogenesis; Protein Processing; Cell Proliferation; Regulated Secretory Pathway; Coagulation; Insulin Secretion; Cell Death |
|---|
| Background | Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance |
|---|
| Scientific References | PMID: 2408021 by van den Ouweland A.M.W., et al. Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2.
PMID: 2251280 by Wise R.J., et al. Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site. |
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Kit Components And Assay QC Data Details Of Human Furin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0916-CAP | 96-well plate precoated with anti-Human FURIN antibody | 1 |
| EK0916-ST | lyophilized recombinant Human FURIN standard | 20ng/tube |
| EK0916-DA | biotinylated anti-Human FURIN antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0916 Human Furin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.015 | 0.055 | 0.096 | 0.177 | 0.346 | 0.704 | 1.343 | 1.882 |
We measured random samples of Human Furin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 417 | 2751 | 82701 | 457 | 2701 | 7870 |
| Standard deviation | 21.68 | 143.05 | 464.4 | 28.79 | 148.55 | 448.59 |
| CV(%) | 5.2% | 5.2% | 5.6% | 5.5%% | 5.5% | 5.7% |
We measured the performance consistency of Human Furin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 417 | 420 | 397 | 384 | 404 | 14.77 | 3.6% |
| Sample 2 | 2751 | 2551 | 2738 | 2985 | 2756 | 153.96 | 5.5% |
| Sample 3 | 8293 | 8993 | 7686 | 9246 | 8554 | 610.96 | 7.1% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all FURIN primary antibodies,
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