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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and cell lysates.|
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Human Furin ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Furin ELISA Kit PicoKine™ (96 Tests). Quantitate Human FURIN in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Furin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0916)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: D108-E715
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0916 is reactive to FURIN in Human samples
Validated Sample Types
cell culture supernatants and cell lysates.
EK0916 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FURIN
Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance
|EK0916-CAP||Anti-Human FURIN Pre-coated 96-well strip microplate||1|
|EK0916-ST||Human FURIN Standard||2 vials, 20 ng/tube|
|EK0916-DA||Human FURIN Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Furin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FURIN, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.
Some articles we found to cite concentrations of FURIN in samples: 22186789 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Furin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FURIN in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FURIN (Source: Uniprot.Org, NCBI)
peptidase S8 family
Dibasic-processing enzyme; EC 3.4.21; EC 18.104.22.168; FUR; FURdibasic processing enzyme; furin (paired basic amino acid cleaving enzyme); Furin; furin, membrane associated receptor protein; PACE; PACEFES upstream region; paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein); Paired basic amino acid residue-cleaving enzyme; PC; PCSK3; PCSK3furin; proprotein convertase subtilisin/kexin type 3; SPC1 FURIN FUR, PACE, PCSK3, SPC1 furin, paired basic amino acid cleaving enzyme furin|FES upstream region|dibasic processing enzyme|furin, membrane associated receptor protein|paired basic amino acid residue-cleaving enzyme|proprotein convertase subtilisin/kexin 3|proprotein convertase subtilisin/kexin type 3|subtilisin-like proprotein convertase 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FURIN, check out the FURIN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FURIN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK0916
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