Human Galectin-1 ELISA Kit PicoKine™

Human Galectin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. Cited in 6 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0762
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Galectin-1 ELISA Kit PicoKine™

See all Galectin-1 products

SKU/Catalog Number

EK0762

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Galectin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Galectin-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0762)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-D135

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0762 is reactive to LGALS1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0762 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0762-CAP Anti-Human LGALS1 Pre-coated 96-well strip microplate 1
EK0762-ST Human LGALS1 Standard 2 vials, 10 ng/tube
EK0762-DA Human LGALS1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0470.1190.1440.2520.5160.8921.6092.092

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Galectin-1, Dilution ratio of 1:1, concentration in serum is around 4ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Galectin-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2321786564722918225807
Standard deviation13.45121.44355.7613.96147.58476.17
CV (%)5.8%6.8%8.1%6.1%8.1%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Galectin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12322262302212274.21.8%
Sample 21786159217461838174091.735.2%
Sample 356475665607352995671274.134.8%
*number of samples for each test n=16.

Gene/Protein Information For LGALS1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09382

Gene ID

3956

Gene Name

LGALS1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LGALS1, check out the LGALS1 Infographic

LGALS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LGALS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0762 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sherief,L.M.,Abd El-khalek,E.R.,Libda,I.A.,Gaber,O.A.,Kamal,N.M.,Soliman,B.K.,Mokhtar,W.A.,Mokhtar,G.A.,Salah,H.E.,Kamar,G.M.,& Zakaria,M.(2021).Serum endocan and endothelial dysfunction in childhood acute lymphoblastic leukemia survivors: a tertiary center experience.Therapeutic Advances in Chronic Disease.https://doi.org/10.1177/20406223211015963
Species: Human
EK0762 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Maternal serum galectin-1 and galectin-3 levels in pregnancies complicated with preterm prelabor rupture of membranes

Expression of immune checkpoint molecules in endometrial carcinoma

Galectin-1 from cancer-associated fibroblasts induces epithelial?mesenchymal transition through ?1 integrin-mediated upregulation of Gli1 in gastric cancer

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma

Tang D, Yuan Z, Xue X, Lu Z, Zhang Y, Wang H, Chen M, An Y, Wei J, Zhu Y, Miao Y, Jiang K. Int J Cancer. 2012 May 15;130(10):2337-48. Doi: 10.1002/Ijc.26290. Epub 2011 Nov 28. High Expression Of Galectin-1 In Pancreatic Stellate Cells Plays A Role...

Have you used Human Galectin-1 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

1 Reviews For Human Galectin-1 ELISA Kit PicoKine™

0

"Works Well For Detection Of Gal-1 Over A Broad Range Of Concentration"

Excellent

Source: Biocompare.com

"Works Well For Detection Of Gal-1 Over A Broad Range Of Concentration"

--Mahesh Raundhal, Weill Cornell Medicine, Medicine, Postdoctoral Fellow

Applications ELISA
Sample Human ovarian cancer ascites
Detection --

"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")

Verified customer

Submitted

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

9 Customer Q&As for Human Galectin-1 ELISA Kit PicoKine™

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Galectin-1 in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-09-23

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-09-23

Question

Does EK0762 react with mouse?

Verified customer

Asked: 2020-06-23

Answer

Our lab tested recombinant mouse Galectin-1 protein using EK0762 Human Galectin-1 ELISA Kit and approximately 9.1% cross reactivity was observed.

Boster Scientific Support

Answered: 2020-06-24

Question

Q: which procedure should I follow in order to thaw whole blood sample for Galectin-1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-03-15

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Galectin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-03-15

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-12-25

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

A. Anand

Verified customer

Asked: 2019-11-11

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-11

Question

Q: What is the optimal O.D. value for Galectin-1 ELISA kit? I used your Galectin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Galectin-1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-1 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Galectin-1 that can be declared/interpreted as positive by the above standard is 15pg/ml.

Boster Scientific Support

Answered: 2018-02-23

Question

i want to buy human galectin 1 elisa kit but my query is that can it work for serum samples that are approximately 14 months old.

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

We can not guarantee the kit will work for this long storaged serum samples. We suggest the customer run a pilot test first.

Boster Scientific Support

Answered: 2017-07-26

Question

Q: can I use citrate plasma as samples in Human Galectin-1 Picokine® ELISA Kit (Catalog # EK0762)?

H. Miller

Verified customer

Asked: 2016-09-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Galectin-1 causing degradation of its protein structure. Galectin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Galectin-1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-09-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Nelson

Verified customer

Asked: 2016-01-22

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-01-22

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0762
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.