Human Galectin-4 ELISA Kit PicoKine™

Human Galectin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1275
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Galectin-4 ELISA Kit PicoKine™

See all Galectin-4 products

SKU/Catalog Number

EK1275

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Galectin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Galectin-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1275)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-I323

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1275 is reactive to LGALS4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1275 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1275-CAP Anti-Human LGALS4 Pre-coated 96-well strip microplate 1
EK1275-ST Human LGALS4 Standard 2 vials, 10 ng/tube
EK1275-DA Human LGALS4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0220.0810.1690.2860.5321.1511.5971.880

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Galectin-4, Dilution ratio of 1:1, concentration in serum is NO-50pg/ml around 300pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Galectin-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6024387964223865
Standard deviation3.2419.1945.74.2819.447.57
CV (%)5.4%7.9%5.2%6.7%8.7%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Galectin-4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 160546064593.576%
Sample 22432582582602546.832.6%
Sample 387992384897890748.895.3%
*number of samples for each test n=16.

Gene/Protein Information For LGALS4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P56470

Gene ID

3960

Gene Name

LGALS4

Full Name

Galectin-4

Weight

35.941kDa

Alternative Names

Antigen NY-CO-27; GAL4; gal-4; galectin 4; Galectin4; Galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4; LGALS4 LGALS4 GAL4, L36LBP galectin 4 galectin-4|L-36 lactose-binding protein|antigen NY-CO-27|gal-4|lactose-binding lectin 4|lectin, galactoside-binding, soluble, 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LGALS4, check out the LGALS4 Infographic

LGALS4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LGALS4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1275 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The roles of galectin-3 and galectin-4 in the idiopatic Parkinson disease and its progression

Have you used Human Galectin-4 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human Galectin-4 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

10 Customer Q&As for Human Galectin-4 ELISA Kit PicoKine™

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

W. Roberts

Verified customer

Asked: 2020-04-19

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-04-19

Question

When does Human Galectin-4 ELISA Kit PicoKine expire after the receipt date?

Verified customer

Asked: 2019-12-03

Answer

The expiration date of a Human Galectin-4 ELISA Kit PicoKine will be 6 months from the manufacturing date if stored at 4°C and 1 year if stored at -20°C.

Boster Scientific Support

Answered: 2019-12-03

Question

Could you please help me to answer a question from one of my customers about your product? See below for further details. We bought your Gal-4 ELISA kit and now with one larger set of cell culture supernatants (52 samples) of different Cell types tested. Unfortunately, all readings were under the blank. We have seen that this ELISA has no references yet. We have a clear question: how much Gal-4 is from primary Chondrocytes secreted. At the moment we are trying to concentrate To increase Gal-4 by increasing the number of cells and the medium so keep it low as possible.Our preliminary data show that Gal-4 is synthesized in our cells.

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

Please suggest the customer follow the two tips listed below to improve the assay. 1. Use diluiton of 1:1 or undiluted samples 2. The cell concentation is recommended to be 106/ml. If the customer had run the assay with the above mentioned samples and obtained blank data, which indicated that the expression level of Gal-4 in primary Chondrocytes was too low to be detected. Gal-4 concnetration in serum and plasma is very low and we are suspecting that the primary Chondrocytes doesn't contain too much Gal-4.

Boster Scientific Support

Answered: 2019-03-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-01-07

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-01-07

Question

Q: can you recommend the dilution ratio of serum samples for detection of Galectin-4 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-12-09

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-12-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-08-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-08-21

Question

Q: Can Galectin-4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

A. Johnson

Verified customer

Asked: 2018-06-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-06-11

Question

Q: can I use citrate plasma as samples in Human Galectin-4 Picokine® ELISA Kit (Catalog # EK1275)?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Galectin-4 causing degradation of its protein structure. Galectin-4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Galectin-4 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-06-28

Question

Q: if the enzyme conjugated Galectin-4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Galectin-4 antigen?

Verified Customer

Verified customer

Asked: 2017-04-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-04-11

Question

Q: What is the optimal O.D. value for Galectin-4 ELISA kit? I performed your Galectin-4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Galectin-4 even though the O.D. values are not very high?

W. Thompson

Verified customer

Asked: 2015-08-21

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-4 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Galectin-4 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2015-08-21

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1275
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.