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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human Galectin-4 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Galectin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1275)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A2-I323
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1275 is reactive to LGALS4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1275 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-4
Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
|EK1275-CAP||Anti-Human LGALS4 Pre-coated 96-well strip microplate||1|
|EK1275-ST||Human LGALS4 Standard||2 vials, 10 ng/tube|
|EK1275-DA||Human LGALS4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Galectin-4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Galectin-4, Dilution ratio of 1:1, concentration in serum is NO-50pg/ml around 300pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Galectin-4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LGALS4 (Source: Uniprot.Org, NCBI)
Antigen NY-CO-27; GAL4; gal-4; galectin 4; Galectin4; Galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4; LGALS4 LGALS4 GAL4, L36LBP galectin 4 galectin-4|L-36 lactose-binding protein|antigen NY-CO-27|gal-4|lactose-binding lectin 4|lectin, galactoside-binding, soluble, 4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LGALS4, check out the LGALS4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1275 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The roles of galectin-3 and galectin-4 in the idiopatic Parkinson disease and its progression
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