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Product Info Summary
| SKU: | EK1275 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Galectin-4 ELISA Kit PicoKine®
View all Galectin-4 ELISA kits
SKU/Catalog Number
EK1275
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Galectin-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LGALS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1275)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A2-I323
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1275 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1275 is reactive to LGALS4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1275 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-4
Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1275-CAP | Anti-Human LGALS4 Pre-coated 96-well strip microplate | 1 |
| EK1275-ST | Human LGALS4 Standard | 2 vials, 10 ng/tube |
| EK1275-DA | Human LGALS4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.022 | 0.081 | 0.169 | 0.286 | 0.532 | 1.151 | 1.597 | 1.880 |
Data Example Images
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Human Galectin-4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Galectin-4, For serum, dilution ratio is 1:1 and concentration is 0-50 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is around 300 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 243 | 879 | 64 | 223 | 865 |
| Standard deviation | 3.24 | 19.19 | 45.7 | 4.28 | 19.4 | 47.57 |
| CV (%) | 5.4% | 7.9% | 5.2% | 6.7% | 8.7% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Galectin-4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 54 | 60 | 64 | 59 | 3.57 | 6% |
| Sample 2 | 243 | 258 | 258 | 260 | 254 | 6.83 | 2.6% |
| Sample 3 | 879 | 923 | 848 | 978 | 907 | 48.89 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For LGALS4 (Source: Uniprot.Org, NCBI)
Gene Name
LGALS4
Full Name
Galectin-4
Weight
35.941kDa
Alternative Names
Antigen NY-CO-27; GAL4; gal-4; galectin 4; Galectin4; Galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4; LGALS4 LGALS4 GAL4, L36LBP galectin 4 galectin-4|L-36 lactose-binding protein|antigen NY-CO-27|gal-4|lactose-binding lectin 4|lectin, galactoside-binding, soluble, 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LGALS4, check out the LGALS4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Galectin-4 ELISA Kit PicoKine® (EK1275)
Hello CJ!
EK1275 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The roles of galectin-3 and galectin-4 in the idiopatic Parkinson disease and its progression
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Human Galectin-4 ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Roberts
Verified customer
Asked: 2020-04-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-19
Question
When does EK1275 expire after the receipt date?
Verified customer
Asked: 2019-12-03
Answer
The expiration date of a Human Galectin-4 ELISA Kit PicoKine will be 6 months from the manufacturing date if stored at 4°C and 1 year if stored at -20°C.
Boster Scientific Support
Answered: 2019-12-03
Question
Could you please help me to answer a question from one of my customers about your product? See below for further details. We bought your Gal-4 ELISA kit and now with one larger set of cell culture supernatants (52 samples) of different Cell types tested. Unfortunately, all readings were under the blank. We have seen that this ELISA has no references yet. We have a clear question: how much Gal-4 is from primary Chondrocytes secreted. At the moment we are trying to concentrate To increase Gal-4 by increasing the number of cells and the medium so keep it low as possible.Our preliminary data show that Gal-4 is synthesized in our cells.
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
Please suggest the customer follow the two tips listed below to improve the assay. 1. Use diluiton of 1:1 or undiluted samples 2. The cell concentation is recommended to be 106/ml. If the customer had run the assay with the above mentioned samples and obtained blank data, which indicated that the expression level of Gal-4 in primary Chondrocytes was too low to be detected. Gal-4 concnetration in serum and plasma is very low and we are suspecting that the primary Chondrocytes doesn't contain too much Gal-4.
Boster Scientific Support
Answered: 2019-03-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-07
Question
Q: can you recommend the dilution ratio of serum samples for detection of Galectin-4 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-12-09
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Galectin-4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-08-21
Question
Q: Can Galectin-4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
A. Johnson
Verified customer
Asked: 2018-06-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-11
Question
Q: can I use citrate plasma as samples in Human Galectin-4 Picokine® ELISA Kit (Catalog # EK1275)?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Galectin-4 causing degradation of its protein structure. Galectin-4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Galectin-4 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-06-28
Question
Q: if the enzyme conjugated Galectin-4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Galectin-4 antigen?
Verified Customer
Verified customer
Asked: 2017-04-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-04-11
Question
Q: What is the optimal O.D. value for Galectin-4 ELISA kit? I performed your Galectin-4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Galectin-4 even though the O.D. values are not very high?
W. Thompson
Verified customer
Asked: 2015-08-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Galectin-4 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Galectin-4 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2015-08-21
