Product Info Summary
SKU: | M08468 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-GAL4 Monoclonal Antibody
View all Galectin-4 Antibodies
SKU/Catalog Number
M08468
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAL4 Monoclonal Antibody catalog # M08468. Tested in WB, IHC applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAL4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08468)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACGG-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAL4 Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M08468 is reactive to LGALS4 in Human, Rat
Applications
M08468 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
35.941kDa
Background of Galectin-4
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GAL4 expression in rat small intestine lysate.
Protein Target Info & Infographic
Gene/Protein Information For LGALS4 (Source: Uniprot.org, NCBI)
Gene Name
LGALS4
Full Name
Galectin-4
Weight
35.941kDa
Alternative Names
Antigen NY-CO-27; GAL4; gal-4; galectin 4; Galectin4; Galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4; LGALS4 LGALS4 GAL4, L36LBP galectin 4 galectin-4|L-36 lactose-binding protein|antigen NY-CO-27|gal-4|lactose-binding lectin 4|lectin, galactoside-binding, soluble, 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGALS4, check out the LGALS4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGALS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAL4 Monoclonal Antibody (M08468)
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No publications found for M08468
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-GAL4 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-GAL4 Monoclonal antibody (M08468)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We do provide the blocking peptide for product anti-GAL4 Monoclonal antibody (M08468). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-16
Question
I see that the anti-GAL4 Monoclonal antibody M08468 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-GAL4 Monoclonal antibody for WB for human colon skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon skin identification?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
As indicated on the product datasheet, M08468 anti-GAL4 Monoclonal antibody has been validated for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-19
Question
Does anti-GAL4 Monoclonal antibody M08468 work on pig WB with colon carcinoma?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
Our lab technicians have not tested anti-GAL4 Monoclonal antibody M08468 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GAL4 Monoclonal antibody M08468 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig colon carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-06
Question
Is this M08468 anti-GAL4 Monoclonal antibody reactive to the isotypes of LGALS4?
M. Zhang
Verified customer
Asked: 2016-04-27
Answer
The immunogen of M08468 anti-GAL4 Monoclonal antibody is A synthesized peptide derived from human GAL4 Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-27
Question
Do you have a BSA free version of anti-GAL4 Monoclonal antibody M08468 available?
E. Mitchell
Verified customer
Asked: 2013-04-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAL4 Monoclonal antibody M08468 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-04